Yeast DNA-dependent RNA Polymerase I A RAPID PROCEDURE FOR THE LARGE SCALE PURIFICATION OF HOMOGENEOUS ENZYME*
Reads0
Chats0
TLDR
Yeast polymerase I separates into two forms when subjected to gel electrophoresis under nondenaturing conditions, the main component which migrates faster contains all the subunits except the polypeptides Ic and If.About:
This article is published in Journal of Biological Chemistry.The article was published on 1976-03-10 and is currently open access. It has received 57 citations till now. The article focuses on the topics: Polymerase & Gel electrophoresis.read more
Citations
More filters
Journal ArticleDOI
Eukaryotic RNA polymerases.
TL;DR: This review will attempt to cover the present information on the multiple forms of eukaryotic DNA-dependent RNA polymerases, both at the structural and functional level.
Journal ArticleDOI
Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II.
H G Hodo rd,S P Blatti +1 more
TL;DR: DNA-dependent RNA polymerase II from calf thymus has been successfully purified using polythylenimine precipitation, which eliminates the high salt extraction of chromatin previously used in purification of this enzyme and makes possible the large scale preparation of mammalian RNA polymerases II.
Journal ArticleDOI
Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
Graeme I. Bell,L.J. DeGennaro,D.H. Gelfand,Robert J. Bishop,Pablo Valenzuela,William J. Rutter +5 more
TL;DR: The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed and the polarity of the 35 S ribosome RNA precursor has been established by a combination of hybridization analysis and DNA sequence determination.
Journal ArticleDOI
Interaction of nucleolar phosphoprotein B23 with nucleic acids.
TL;DR: These studies indicate that protein B23 binds cooperatively with high affinity for single-stranded nucleic acids and exhibits RNA helix destabilizing activity, which may be related to its role in ribosome assembly.
Journal ArticleDOI
Association of protein C23 with rapidly labeled nucleolar RNA
Amy H. Herrera,Mark O.J. Olson +1 more
TL;DR: The association of nucleolar phosphoprotein C23 with preribosomal ribonucleoprotein (RNP) particles was examined in Novikoff hepatoma nucleoli and Dot-blot hybridizations indicated that the RNA in the RL fraction and the immunoprecipitated RNA contained sequences from 18S and 28S ribosomal RNA.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Journal ArticleDOI
Multiple Forms of DNA-dependent RNA Polymerase in Eukaryotic Organisms
TL;DR: Three distinct RNA polymerase activities have been isolated from developing sea urchin embryos, and in rat liver nuclei there are two RNA polymerases activities.