Showing papers on "Potassium dichromate published in 2019"

Barium titanate nanostructures for photocatalytic hydrogen generation and photodegradation of chemical pollutants

Abstract: Barium titanate nanoparticles (NPs) were synthesised using a modified sol–gel technique. The structure and morphology of NPs were described using various techniques. The photocatalytic activities of the NPs were evaluated by the photocatalytic degradation of Eriochrome black T and potassium dichromate in the presence of UV light irradiation. The barium titanate NP catalyst exhibited higher photocatalytic activity for the degradation of pollutants effectively at room temperature. The different parameters effects such as pollutant initial concentration, loading of photocatalyst, initial pH values of the solution were also examined on the decolourization efficiency of the pollutants. The highest degradation efficiency was achieved for Eriochrome dark T (93%) and potassium dichromate (92%) pollutants. The prepared NPs showed 26 μmol g−1 hydrogen generation within 5 h.

Oxidative Effects of Potassium Dichromate on Biochemical, Hematological Characteristics, and Hormonal Levels in Rabbit Doe (Oryctolagus cuniculus).

Abstract: The present study was conducted to evaluate the toxicity induced by the increasing doses of potassium dichromate in rabbit doe. Twenty-eight adult does of 6 months of age were divided into four groups (A, B, C, and D; n = 7), with comparable average body weight (bw). Group A rabbits received only distilled water daily and served as a control, while groups B, C, and D received, respectively, 10 mg/kg bw, 20 mg/ kg bw, and 40 mg/kg bw of potassium dichromate via gavage for 28 days, after which animals were anesthetized with ether vapor and sacrificed. Blood samples were obtained via cardiac puncture and collected without anticoagulant for biochemical dosages and with anticoagulant (EDTA) for complete blood count. Follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) were dosed in serum and in homogenates of ovary with the help of AccuDiagTM ELISA kits from OMEGA DIAGNOSTICS LTD (Scotland, England) while respecting the immuno-enzymatic method. Activities of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and concentration of malondialdehyde (MDA) in liver, kidney, ovary and uterus were measured. Hematology revealed a significant (p 0.05) difference was registered in monocytes, red blood cells, hematocrits, and plaquetocrits values with respect to the control. No matter the organ considered, no significant (p > 0.05) change was recorded in weight and volume. Nephrotoxicity analysis registered a significant (p 0.05) variation in aspartate aminotransferase but total protein, alanine aminotransferase, and total cholesterol increased significantly (p 0.05) difference in glutathione reductase except in ovary where a significant (p 0.05) change. Superoxide dismutase and malondialdehyde recorded a significant (p < 0.05) decrease and increase respectively, with respect to the control. Results obtained from this study showed that the reduction process of chromium in tissues may cause the generation of reactive oxygen species, which are involved in hematoxic, nephrotoxic, hepatotoxic, and reproductive toxicity effects.

Bioreduction of toxic hexavalent chromium by novel indigenous microbe Brevibacillus agri isolated from tannery wastewater

Abstract: The present study is focused on the microbial bioreduction of hexavalent chromium generated from tannery waste. For the study, the treated and untreated tannery effluents were collected from an unexplored site of Manpura, Macheri, Jaipur (India). A few bacterial strains capable of tolerating and reducing hexavalent chromium were isolated, and the most capable one that reduced 85% of hexavalent chromium was identified to be Brevibacillus agri by 16S rDNA gene sequencing. This isolate could tolerate high concentrations of chromium up to 850 mg/l of potassium dichromate and showed maximum reduction of about 85% for 100 mg/l of hexavalent chromium at temperature 37 °C and pH 7.0 in 48 h. The potential of the partially purified enzyme to reduce hexavalent chromium was measured by diphenyl carbazide assay. Purified enzyme showed 0.26 units bioactivity to reduce Cr, in the presence of reduced nicotinamide adenine dinucleotide and Km and Vmax were observed to be 1.9 mM and 0.045 mM/min (mg protein), respectively. The current study, therefore, showed Brevibacillus agri capable of reducing hexavalent chromium without usage of toxic chemicals and, henceforth, prevented generation of harmful by-products.

Renal Protective Effects of Coenzyme Q10 Against Chromate Induced Nephrotoxicity in Rats

Abstract: Background and Objective: Exposure to human carcinogens as hexavalent chromate compounds is unavoidable. Chromate induces nephrotoxicity mainly due to increased cellular oxidative stress. The current study evaluates the renoprotective effects of coenzyme Q10 (CoQ10) in potassium dichromate (Chromate) induced nephrotoxicity in rats. Materials and Methods Animals were divided to 3 groups, normal control group was fed distilled water, positive control group was treated by 12 mg kgG1 chromate once per week for 6 weeks. The third group was treated daily by CoQ10 (10 mg kgG1) for 6 weeks and 12 mg kgG1 chromate once per week for 6 weeks. At the end, blood pressure (BP) and heart rate (HR) were measured. Kidney function tests, lipid profile, oxidative stress and inflammatory bio-markers were determined. Results Chromate resulted in hypertension, worsens kidney function tests, oxidative stress and inflammatory bio-markers. The use of CoQ10 ameliorated these harmful effects. This could be attributed to its antioxidant and anti-inflammatory activity. Conclusion: The present results suggest that CoQ10 has a promising potential in the protection against chromate-induced nephrotoxicity.

Effects of Chromium on Enzymatic/Nonenzymatic Antioxidants and Oxidant Levels of Portulaca oleracea L.

Abstract: Purslane(Portulaca oleracea L.) is a local plant in Iran that can easily breed. Chromium is a heavy metal that causes toxicity for the growth and development of plants. To study the effects of potassium dichromate on growth, efficiency of photosystem II (Fv/Fm), cell membrane stability index (MSI), indexes of oxidative stress and antioxidant properties of purslane, an experiment was done in a completely randomized design of five levels (0, 25, 50, 75 and 100 ppm Cr per kg of soil) in a greenhouse condition. After 60 days, the effect of different levels of chromium on growth parameters, Fv/Fm, MSI, total phenolic compound, flavonoid, H2O2 and malondialdehyde content and antioxidants enzymatic activity (catalase, peroxidese and ascorbate peroxidase) was assayed. Along With increasing concentrations of chromium, the malondialdehyde (MDA) and H2O2 was increased, and followed by the enzymatic and nonenzymatic antioxidant compounds (except APX) was increased to compensate the effect of oxidant compounds. But, Heavy metal negatively affected growth parameters such as dry weight and length of root and shoot, Fv/Fm and MSI in contaminated plants. Therefore, the destructive effects due to chromium stress with increases the activity of enzymatic and non-enzymatic antioxidant system was minimized. It was noted that accumulation of chromium in the roots was higher than the shoots of the plants under treatment, significantly. Based on these results, purslane can be introduced as a good candidate for tolerance to chromium.

Analytic method for copper-ferric continuous determination in mineral

Abstract: The invention discloses an analytic method for copper-ferric continuous determination in mineral. The method comprises the following steps: separating ferric from copper by adopting ammonium hydroxide, precipitating ferric hydroxide, adding hydrochloric acid to dissolve, determining the ferric by adopting a potassium dichromate titration, and determining the copper by using iodometry after treating the separated copper ammonia solution through the hydrochloric acid and the nitric acid. Through the method disclosed by the invention, two elements are determined by once dissolving a sample, the advantage is that the copper and the ferric are separately determined and are non-interference; the mutual influence is avoided, and the experiment proof method is feasible. The analytic method disclosed by the invention is suitable for the mineral sample with copper-ferric content, namely mass percent greater than 1%, in the mineral.

Protective Effect of Curcuma Against Chromium Hepatotoxicity in Rats

Abstract: This study was carried out to investigate the antioxidant effects of curcuma against chromium-induced alterations in hepatic indices and dysfunctions in the antioxidant system. Forty male Wistar rats were randomly divided into four groups and were treated for 30 consecutive days. The control group (0-0) received per os mineral water and normal diet. The second group (0-Cur) received mineral water and an experimental diet containing 2% of curcuma powder, whereas the third group (Cr-0) was orally fed (per os) with 15 mg/kg body weight/day of potassium dichromate and normal diet. The last group (Cr-Cur) received per os 15 mg/kg of potassium dichromate and a diet with 2% of curcuma. The treatment by chromium was found to elicit a perturbation in biochemical parameters producing a significant increase in glycemia, triglycerides, cholesterol, ALP, ALT, AST, and LDH levels. On the contrary, a significant reduction was observed in the oxidative stress-related parameters (GSH, GPx, CAT, and GST). Moreover, we noticed that liver sections of rats intoxicated with chromium showed a disrupted architecture. However, the administration of curcuma revealed an intense reduction in the oxidative stress induced by chromium, ameliorating the levels of the majority of the previous parameters. The data of this study revealed the potent antioxidant effects of curcuma in reducing oxidative stress damage induced by the hexavalent chromium.

Gun bore cleaning compound and preparation method thereof

Abstract: The invention provides a gun bore cleaning compound and a preparation method thereof. The gun bore cleaning compound uses a high-molecular complexing agent, a foaming agent and the like as raw materials; the prepared gun bore cleaning agent does not contain carcinogenic substances such as Cr ions, is free of harm to the bodies of cleaning officers and soldiers, is friendly to environment, and canreplace the environment-unfriendly heavy-smell cleaning compounds containing ammonium carbonate and potassium permanganate or potassium dichromate solutions. The gun bore cleaning compound is free ofdamage to the human body and free of pollution to the environment.

Sub-lethal effects of potassium dichromate on hematological and histological parameters in climbing perch, Anabas testudineus (Anabantidae)

Abstract: Chromium, which enters the river through anthropogenic sources, is one of the potent heavy metals. The present study is an attempt to determine the LC 50 of Potassium dichromate for the climbing perch, Anabas testudineus and to study the impact of two sub-lethal concentrations (6 and 12 mg/l) of Potassium dichromate the toxic hexavalent Cr(VI) form of Chromium on this fish through investigating hematological and histopathological parameters. Experimental set up included quadruplicate treatments for each dosage, and the results were compared with control treatments. The results showed that the LC 50 value at 96 hr was 59.92 mg/l. The fishes exposed to sub-lethal concentrations showed severe abnormalities such as; degeneration of hepatocytes, necrosis of hepatic tissue and extensive haemorrhage in gills and renal tissue. The present study brings out the harmful impact of Cr(VI) in the aquatic environment and necessitates regulations of its inflow to natural water bodies as a management plan to curb its contamination.

Bioremediation Efficacy of Extracellular Chromate Reductase from Bacillus amyloliquefaciens (CSB 9) for Detoxification of Hexavalent Chromium

Abstract: Extracellular Cr(VI) reductase was produced by a chromate resistant bacterium Bacillus amyloliquefaciens with optimized physico-chemical parameters. Subsequently, the purified Cr(VI) reductase showed specific activity of 0.167 U/mg, 6% yield and 30.36-fold increase in purity. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight (M) of the purified enzyme of interest was estimated to be ~116 KDa. The purified enzyme was further subjected for functional characterization (influence of pH, temperature and storage stability). The Cr(VI) reductase activity of the purified enzyme for temperature and pH optima was at 35 °C and 7.0 respectively on standard analysis conditions. Using potassium dichromate as substrate, the enzyme showed maximum activity (Vmax) of 3.5 U/mL with its corresponding KM value of 27.78 μM. The purified enzyme exhibited higher stability when treated with certain additives. These remarkable qualities found with this enzyme produced by B. amyloliquefaciens could make this an ideal candidate for bioremediation of Cr(VI) under a wide range of environmental conditions.

Adverse Chromate Reactions on the Skin

Abstract: Chrome ulcers were first described by William Cumin, in Glasgow 1827, in two dyers and in a person involved in the manufacture of potassium bichromate. He used potassium bichromate locally as a treatment for tuberculosis and found this produced papules which became pustules and ulcerated. It is essential to understand that allergy to chromium metal does not occur as in nickel sensitivity, hence potassium dichromate is the standard material for patch testing sensitivity to chromate compound ion. Patch testing with trivalent chromium does not produce such a high percentage of positives as hexavalent chromium. Anderson found that none of the patients who gave a positive patch test to potassium dichromate were positive to chromium chloride. A positive patch test will consist of redness with some induration; severe reactions will produce a definite eczematous response. Many metals, e.g., nickel, cobalt, and chromate can produce pustular reactions which do not indicate allergy.

Effects of chronic exposure to hexavalent chromium in water on oxidative stress parameters in Wistar rats

Abstract: Hexavalent chromium [Cr (VI)] is a metal utilized in different industries and consequently disposed in the environment. It is a toxic substance and its reduction to trivalent Cr [Cr (III)] generates intermediates, which are responsible for the oxidation of molecules, and cause the oxidative stress. Therefore, the aim of this study was to evaluate if Cr (VI) could induce oxidative stress in Wistar rats. In this study, Wistar rats were chronically exposed to 25 and 50 ppm of potassium dichromate in drinking water for 30 days. The levels of Cr were evaluated in the blood and tissues (liver, kidneys, and lungs). Oxidative stress was determined in the liver, kidneys, and lungs and was evaluated by DFCH, TBA-RS and carbonyl test. Antioxidant enzymes were evaluated through catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. Regarding the results, Cr concentration was significantly elevated in all tissues, however, it was lower in the lungs. In relation to the oxidative stress parameters, there was a significant increase of DCFH levels in the kidneys and carbonyls in liver and kidneys. Regarding the antioxidant enzymes, SOD was decreased in all organs and GPx was diminished in the kidneys. These data indicated that Cr (VI) could induce oxidative stress in the kidneys and liver due to an imbalance between oxidative and antioxidant parameters. The lungs were little affected, possibly by the lowest chromium accumulation.

Fourier holograms in grenetina doped with potassium dichromate

Abstract: The recording of Fourier Holograms in a photosensitive material composed of the mixture of 4% of grenetine, 1% of potassium dichromate and 95% of water is presented. This mixture is deposited in a cell formed by two slides separated by a plastic film of 0.01 mm thickness; the recording was carried out with a beam of light from an Ar laser with a wavelength of 532 nm. Some experimental results are shown.

Acute Toxicity of Herbicides and Sensibility of Aquatic Plant Wolffia brasiliensis as a Bioindicator Organism

Abstract: This study aimed to establish the mass cultivation of Wolffia brasiliensis under laboratory conditions, determine the sensitivity of reference substances (potassium dichromate, sodium chloride, and potassium chloride), and estimate the acute toxicity (LC50;7d) for the herbicide glyphosate, diquat, paraquat, imazapyr, 2,4-D, saflufenacil, imazamox, penoxsulam, metsulfuron-methyl, and atrazine. Three media were tested for culture establishment: Hoagland’s medium, 5.0 g L-1 NPK fertilizer (20-5-20), a mixture of Oxisol + organic substrate of decaying aquatic plant (2:1; v v-1). Sensitivity tests with reference substances and acute toxicity for herbicides were carried out after establishing the cultivation. The species W. brasiliensis presented an excellent sensitivity response to reference substances, with means of LC50;7d of 15.61±2.13 g L-1, 17.02±0.50 g L-1, and 52.54±4.27 mg L-1 for sodium chloride, potassium chloride, and potassium dichromate, respectively. The acute toxicity response indicated that W. brasiliensis was extremely sensitive to diquat (LC50;7d = 0.07 mg L-1) and paraquat (LC50;7d < 0.05 mg L-1), sensitive to imazapyr (LC50;7d = 21.10 mg L-1), and little sensitive to the other herbicides. The response capacity for the different groups of herbicides, the standardized response to reference substances, and ease of cultivation in the laboratory indicate the potential for using this aquatic plant in the assessment of herbicide risk in aquatic environments.

Method for measuring organic matter content of organic fertilizer

Abstract: The invention relates to a method for measuring the organic matter content of an organic fertilizer and belongs to the field of organic fertilizer quality detection. According to the method, the amount of a sample is determined at first, and then detection is carried out; and therefore, the dilution and constant volume process of a potassium dichromate concentrated sulfuric acid oxidation reactionsolution are eliminated, the time of the operation of the potassium dichromate concentrated sulfuric acid solution is significantly reduced, measurement efficiency is improved, and the risk of measurement can be reduced; and a condition that re-measurement is required when a user finds that potassium dichromate is incompletely oxidized in the last step of titration in a commonly used measurementmethod can be avoided.

Non-fluorescent filters for fluorescence detection with in-line geometry.

Abstract: Intrinsic emission from typical filters can unexpectedly contribute to the total measured signal in a fluorescence system. This emission becomes even more problematic for in-line geometry measurements where the excitation light can directly excite the emission filter. Potassium dichromate has minimal intrinsic fluorescence even with ultra-violet (UV) excitation. We show that a liquid sample can be tuned for its transmission properties by adding a base to the solution. This makes it attractive for use as an optical filter. In addition, when embedded in poly (vinyl alcohol) (PVA) film, potassium dichromate is even less fluorescent. These films can be cut and molded into practically any size or shape. Thus non-fluorescent, modular and cheap filters composed of potassium dichromate embedded in a PVA film is proposed to eliminate this unwanted emission and is suitable for a wide variety of devices and applications.

Pharmacological Profile of Diospyros melanoxylon Methanolic Extract

Abstract: Present study was aimed to investigate the in-vitro antioxidant, anti-inflammatory and in-vivo nephroprotective activity novel herbal extracts. Initially the test extracts were evaluated for antioxidant potential by performing the DPPH assay. Few extracts displayed potent antioxidant activity by DPPH free radical scavenging activity. Among all the extracts Methanolic extracts of Diospyros melanoxylon was found to be more potent for antioxidant potential. Presence of phenols and flavonoid in the test extracts might be contributed to their potent antioxidant activity. The test extracts were also evaluated for anti-inflammatory activity by carrageenan induced paw edema model. RX- has shown moderate anti-inflammatory activity. Based on preliminary in-vitro antioxidant activity results Diospyros melanoxylon was selected for further to evaluate Nephro-protective activity against Potassium dichromate induced model. Acute oral toxicity test was performed to find out the safe dose of test extract before going to in vivo evaluation (Potassium Dichromate induced nephrotoxicity). Acute toxicity study of test extract was conducted in wistar rats to find the Maximum tolerated dose. The test extract did not show any toxicity and mortality symptoms during the study at the different doses studied. The Maximum Tolerated Dose (MTD) of the test extract was found to be >2000 mg/kg in rats. In-vivo nephroprotective activity was conducted in wistar rats by Potassium dichromate-induced nephrotoxicity model. During the study period test extract (250 mg/kg, 500 mg/kg) were administered by oral route for the period of 7 days followed by potassium dichromate administration (15 mg/kg). At the end of the study blood samples were collected and used for estimation of kidney biochemical parameters. Results showed that significant increase was observed in biochemical parameters (BUN, CR) in PDC group compared to vehicle control. The test extract displayed significant reduction in blood urea nitrogen and serum creatinine at the dose of 500 mg/kg. Kidney tissue samples were collected on termination day of all rats and subjected for measurement of antioxidant enzymes and lipid Peroxidation to check the organ toxicity. Significant increase in lipid Peroxidation and decrease in antioxidant enzyme levels were observed in PDC control whereas test extract prevented the kidney toxicity by decreasing TBARS production and normalization of antioxidant defense enzymes at the doses studied. Gain in body weight and organ weight compared to PD control also revealed the Nephroprotective effect of D. melanoxylon extract at both the doses. All the data showed that both biochemical antioxidant parameters correlated together and supported the protective effect of the Herbal extract (D. melanoxylon) against potassium dichromate induced nephrotoxicity.

Determination method for K O content in production of potassium dichromate

Abstract: The invention discloses a determination method for K O content in the production of potassium dichromate, comprising the following steps of: taking V of the potassium dichromat reaction liquid to bedetected, adding reducing agents to reduce the hexavalent chromium to trivalent chromium under acidic conditions, adding alkali to neutralize until the solution is neutral, keeping the temperature at90-100 DEG C for 1-2 h, filtering and washing to be free of K , and collecting the washing solution; heating the obtained washing solution to boiling, adding sodium tetraphenylborate solution dropwise under stirring to generate potassium tetraphenylborate precipitates, and cooling and standing; transferring the cooled solution obtained in the step 2 to a G4 glass sand core hopper dried to a constant weight at 120 DEG C to carry out the suction filtration, washing the obtained precipitations with the potassium tetraphenylborate solution, and finally placing into a dryer to dry to the constant weight W; and calculating the K O content as A = 0.1314W1000 / V.

Environment-friendly preparation method of 2-acetylbenzimidazole

Abstract: The invention discloses an environment-friendly preparation method of 2-acetylbenzimidazole. The method comprises steps as follows: 2-alpha-hydroxyethyl benzimidazole is oxidized by air or oxygen in an alcohol water solvent under the action of an alkaline environment catalyst, and 2-acetylbenzimidazole is obtained, wherein the catalyst is a compound of Pd/C and NaBH4 or KBH4, alkali is K2CO3, KOH,Na2CO3 or NaOH, and the alcohol solvent is C1-C5 fatty alcohol. By means of the provided preparation method, common potassium permanganate and potassium dichromate in a conventional process are avoided, operation is simplified and pollution is reduced by means of a water solution reaction at the room temperature and recyclability of the catalyst, and green and clean production is realized while the production cost is reduced.

Method and device for measuring reaction rate constant of chromate-dichromate ions

Abstract: The invention relates to a method and a device for measuring a reaction rate constant of chromate-dichromate ions. The method is used for measuring the reaction rate constant of chromate-dichromate ions by taking a silver nitrate solution as a disturbance liquid and a potassium dichromate solution as a disturbed solution. The device is primarily structured such that a USB communication system connects a pH and temperature measuring device and an upper computer system which measures the reaction rate constant based on an LabVIEW relaxation process acidity meter, wherein the pH and temperature measuring device stretches into a constant temperature water bath device with a stirring function. The method is quick and accurate to test and simple to operate, and the tested constant is in a Van'tHoff rule permitted deviation range. The dosage of potassium dichromate is reduced by over half, so that the method is green and environment-friendly. The disturbance liquid is replaced by the non-toxic silver nitrate solution, so that the toxicity of the solution is further reduced greatly. The device replaces manual recording and processing of data, so that the method is quick and accurate to test and simple to operate.

Organic matter passivated aluminum powder aluminum content detecting method

Abstract: The invention relates to an organic matter passivated aluminum powder aluminum content detecting method. Coating film is firstly dissolved with an organic solvent acetone, then activated aluminum andiron produce redox reaction in an acidic medium and under the protection of CO2 gas, ferric iron is reduced into divalent iron, sodium diphenylamine sulfonate is used as an indicating agent, a potassium dichromate standard solution is titrated, and the active aluminum content is calculated according to the volume of the consumed potassium dichromate standard solution. The active aluminum content of an organic matter passivated aluminum powder finished product is accurately detected. It is ensured that the active aluminum content of the produced product meets the demand of a customer.

Photoreduced hexavalent chromium ion imprinted polymer material, preparation and application thereof

Abstract: Belonging to the field of new materials, the invention discloses a preparation method of a photoreduced hexavalent chromium ion imprinted polymer material According to the method, potassium dichromate is adopted as the template, 2-vinyl pyridine is taken as the functional monomer, trimethylolpropane trimethacrylate and ethylene glycol dimethacrylate are employed as the crosslinking agent, tolueneis used as the pore-foaming agent, and graphene oxide is taken as the solid surfactant, and Pickering emulsion technology is utilized to prepare the hexavalent chromium ion imprinted polymer material Under an illumination condition, graphene oxide provides reduction electrons, hexavalent chromium is reduced by the valence state transformation of electronic mediators (ferric ions) between trivalence and bivalence, and the reduction product trivalent chromium is adsorbed by functional groups on the surface of graphene oxide through charge effect The method combines ion imprinting technology with photocatalysis technology, realizes the concept of integrated adsorption and reduction of hexavalent chromium ions, also can selectively purify hexavalent chromium ions in water, and has no need for secondary treatment of the generated trivalent chromium ions

Calibration method of potassium dichromate standard solution

Abstract: The invention discloses a calibration method of a potassium dichromate standard solution. The calibration method comprises the following steps that S1, a pure iron sample is weighed and is placed in aconical flask, hydrochloric acid and concentrated nitric acid are added slowly; S2, the conical flask is boiled at low temperature, a stannous chloride solution and the hydrochloric acid are continuously added dropwise; S3, the conical flasks cools; S4, water and a sodium tungstate dihydrate solution are added in the conical flask, and a titanium trichloride solution is added dropwise; S5, the standard solution is added dropwise in the conical flask until blue disappears, sulphuric acid-phosphoric acid mixed acid is added immediately, and a diphenylaminesulfonic acid sodium salt indicator solution are added; S6, the potassium dichromate standard solution is titrated into the conical flask until the solution in the conical flask turns into blue-green from green, when the last drop shows astable purple, titration is completed, and the volume V of the potassium dichromate standard solution consumed by titration is recorded; and S7, according to the addition quantity, molar concentrationof the potassium dichromate standard solution can be calculated. The method is accurate in calibration, and is simple and easy to master.

Protective Effect of Agrimonia pilosa var. Extract on Cultured NIH3T3 Fibroblasts Damaged by Potassium Dichromate

Abstract: Received May 15, 2019 Revised May 31, 2019 Accepted May 31, 2019 The protective effect of Agrimonia pilosa var. (AP) extract on potassium dichromate (K2Cr2O7)induced cytotoxicity in cultured NIH3T3 fibroblasts, was examined by performing an XTT assay for the cell viability and antioxidative effects, such as lactate dehydrogenase (LDH) activity and superoxide anion-radical (SAR) scavenging activity. In this study, K2Cr2O7 decreased the cell viability significantly in a dose-dependent manner, and the XTT50 value was determined to be 37.5 M, which was highly-toxic according to the Borenfreund and Puerner’ toxic criteria. The antioxidant, butylated hydroxytoluene (BHT), increased remarkably the cell viability damaged by K2Cr2O7-induced cytotoxicity in these cultures. With regard to the protective effect of the AP extract on K2Cr2O7-induced cytotoxicity, AP extract produced a significant increase in cell viability and antioxidative effects as the inhibitory ability LDH and SAR scavenging ability. These findings suggest that oxidative stress is involved in the cytotoxicity of K2Cr2O7, and the AP extract effectively protected the cells from K2Cr2O7-induced cytotoxicity by antioxidative effects. These results suggest that natural resources, such as AP extract, may be a putative therapeutic agent for the diminution or treatment of cytotoxicity induced by heavy metallic bases, such as K2Cr2O7 correlated with oxidative stress. Copyright © 2019 The Korean Society for Clinical Laboratory Science. All rights reserved.

Method for determining water quality dichromate

Abstract: The invention relates to a method for determining water quality dichromate. The method comprises the steps of determining reagents required to be added, including mercury sulfate solution, potassium dichromate solution, explosion-proof boiling glass beads, silver sulfate-sulfuric acid solution, ferrous iron indicator solution and ammonium ferrous sulfate; and performing an experimental determination comprising the following steps of placing a water sample required to be tested in a conical flask; adding mercury sulfate to the conical flask for shaking; filling the conical flask with nitrogen through the nitrogen supply system; filling the nitrogen supply system with the potassium dichromate solution in an atomized state, and simultaneously adding the silver sulfate-sulfuric acid solution to the conical flask; heating the conical flask to boil the internal reaction reagent, and keeping at a slight boiling reflux for 1 hour from the boiling; titrating the solution in the conical flask with ammonium ferrous sulfate by using the ferrous iron indicator solution as an indicator, and calculating the chemical oxygen demand by a mathematical formula. The method for determining water qualitydichromate can greatly shorten the chemical measurement time.

High-purity iron oxide red powder content detection method

Abstract: The invention discloses a detection method for high-purity iron oxide red powder. The detection method for the high-purity iron oxide red powder is a potassium dichromate method and includes the following steps of: sampling; hydrochloric acid dissolution; titanium tetrachloride reduction; adding potassium dichromate dropwise to a phosphoric and sulfuric acid medium to carry out oxidization until reaching an end point; and data processing. According to the reduction end point, sodium tungstate is adopted as an indicator; according to the oxidization, sodium diphenylaminesulfonate is adopted asan indicator; and acid dissolution is carried out under the condition of boiling; excessive titanium chloride is removed through the potassium dichromate. The method has the advantages of simplicity,efficiency, high reaction sensitivity and fewer toxic substances, and can accurately measure the purity of the iron oxide red powder.