Showing papers on "Sex pheromone published in 2022"

Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda.

Abstract: BACKGROUND The fall armyworm, Spodoptera frugiperda (J. E. Smith), is a polyphagous moth species and spreading all around the globe. It uses (Z)-9-tetradecenyl acetate (Z9-14:Ac) and (Z)-7-dodecenyl acetate (Z7-12:Ac) (100:3.9) as essential sex pheromone components. However, our understanding of the molecular basis of pheromone detection of S. frugiperda is still incomplete. RESULTS Herein, we identified six PRs, i.e. SfruOR6, 11, 13, 16, 56, and 62, by transcriptome sequencing. Subsequently, we heterologously expressed them in Drosophila OR67d neurons and determined their response spectra with a big panel of sex pheromones and analogs. Among them, SfruOR13-expressing neurons strongly respond to the major sex pheromone component, Z9-14:Ac, but also comparably to (Z,E)-9,12-tetradecadienyl acetate (Z9,E12-14:Ac) and weakly to (Z)-9-dodecenyl acetate (Z9-12:Ac). Both SfruOR56 and SfruOR62 are specifically tuned to the minor sex pheromone component, Z7-12:Ac with varying intensities and sensitivities. In addition, SfruOR6 is activated only by Z9,E12-14:Ac, and SfruOR16 by both (Z)-9-tetradecenol (Z9-14:OH) and (Z)-9-tetradecenal (Z9-14:Ald). However, the OR67d neurons expressing SfruOR11 remain silent to all compounds tested, a phenomenon commonly found in the OR11 clade of Noctuidae species. Next, using single sensillum recording, we characterized three sensilla types on the antennae of males, namely A, B, C and D types that are tuned to the ligands of PRs, thereby confirming that S. frugiperda uses both SfruOR56 and SfruOR62 to detect Z7-12:Ac. Last, using wind tunnel assay, we demonstrate that both Z9,E12-14:Ac and Z9-14:OH act as antagonists to the sex pheromone. CONCLUSION We have deorphanized five PRs and characterized four types of sensilla responsible for the detection of pheromone compounds, providing insights into the peripheral encoding of sex pheromones in S. frugiperda.

Optimization of a pheromone lure by analyzing peripheral coding of sex pheromones of Spodoptera frugiperda in China.

Abstract: BACKGOUND Sex pheromones of the fall armyworm, Spodoptera frugiperda, show differences in composition and proportions in different geographical populations, but always contain Z9-14:OAc as the major component. Odorant receptor neurons (ORNs) housed in the long trichoid sensilla (TS) of male antennae are essential to detect female-released sex pheromones in moths. RESULTS In this study, we identified seven components from pheromone gland extracts of female S. frugiperda in the Yunnan population from China., including (Z)-7-dodecen-1-yl acetate (Z7-12:OAc), (Z)-9-tetradecenal (Z9-14:Ald), (Z)-9-dodecen-1-yl acetate (Z9-12:OAc), (Z)-9-tetradecen-1-yl acetate (Z9-14:OAc), (E)-11-tetradecen-1-yl acetate (E11-14:OAc), (Z)-11-tetradecen-1-yl acetate (Z11-14:OAc), and (Z)-11-hexadecen-1-yl acetate (Z11-16:OAc) at a ratio of 1.2:4:0.8:79.1:1.6:1.6:11.7 by gas chromatography coupled with mass spectrometry. Gas chromatography-electroantennographic detection showed that Z9-14:OAc, Z7-12:OAc, and Z11-16:OAc are the male antennal active components. Peripheral coding of pheromones in males is investigated by single sensillum recording. Five functional neurons housed in three types of TS are identified based on profiles of neuronal responses, which are responsible for attractive component Z9-14:OAc, synergistic components Z7-12:OAc, Z11-16:OAc, interspecific pheromones (Z)-9-tetradecen-1-ol (Z9-14:OH) and (Z,E)-9,12-tetradecadien-1-yl acetate (Z9,E12-14:OAc), respectively. Wind tunnel and field tests demonstrated that ternary combination of Z9-14:OAc, Z7-12:OAc, and Z11-16:OAc at the ratio of 88:1:11 shows the strongest attractiveness to males. CONCLUSION An optimized pheromone blend of Z9-14:OAc, Z7-12:OAc, and Z11-16:OAc in a 88:1:11 ratio is identified for monitoring the invasive pest S. frugiperda in China. Five functional ORNs encoding intra- and interspecific pheromones are identified in male antennae, of which, three neurons encode attractive component Z9-14:OAc, synergistic components Z7-12:OAc and Z11-16:OAc, respectively, and the other two neurons encode interspecific pheromones Z9-14:OH and Z9,E12-14:OAc, separately. This article is protected by copyright. All rights reserved.

Olfactory Strategies in the Defensive Behaviour of Insects

Abstract: Simple Summary Insects have several methods to protect themselves and their resources from danger. One of them is to use their sense of smell. In this review, we describe how insects use smell to detect threats and perform behaviours of ‘flight or fight’ such as avoidance, escape or attack, in order to protect themselves. We also discuss how group-living insects share the information of danger through semiochemicals called alarm pheromones, to act as a collective. In the second section of this paper, we review how these odours are processed in insect brains. We discuss how the two kinds of neural architectures observed in olfactory areas, labelled-lines and across-fiber patterns, support the processing of alarm pheromones. Finally, we give an outlook on potential future studies that will help us understand this field better. Abstract Most animals must defend themselves in order to survive. Defensive behaviour includes detecting predators or intruders, avoiding them by staying low-key or escaping or deterring them away by means of aggressive behaviour, i.e., attacking them. Responses vary across insect species, ranging from individual responses to coordinated group attacks in group-living species. Among different modalities of sensory perception, insects predominantly use the sense of smell to detect predators, intruders, and other threats. Furthermore, social insects, such as honeybees and ants, communicate about danger by means of alarm pheromones. In this review, we focus on how olfaction is put to use by insects in defensive behaviour. We review the knowledge of how chemical signals such as the alarm pheromone are processed in the insect brain. We further discuss future studies for understanding defensive behaviour and the role of olfaction.

(Z,E)‐α‐Farnesene – sex pheromone component of female click beetle Selatosomus aeripennis destructor with intra‐ and inter‐sexual communication function

Abstract: Sex pheromones are known for only a few major click beetle (Coleoptera: Elateridae) pests in North America. These pheromones could be used to monitor, as well as control, adult beetles. Our objective was to identify the sex pheromone of female Selatosomus aeripennis destructor (Brown), a predominant elaterid pest species in farmland in the Canadian Prairie provinces. Headspace volatiles from a group of S. a. destructor females were collected and analyzed by coupled gas chromatography‐electroantennographic detection (GC‐EAD) and gas chromatography‐mass spectrometry (GC‐MS). The GC‐EAD recordings revealed two candidate pheromone components that elicited weak (CPC‐1) or strong (CPC‐2) responses from adult male S. a. destructor antennae. The mass spectra of CPC‐1 and CPC‐2 indicated, and authentic standards confirmed, that they were the isomers (E)‐β‐farnesene and (Z,E)‐α‐farnesene, respectively. In three field experiments at various sites in Alberta, Canada, traps baited with synthetic (Z,E)‐α‐farnesene captured 7.1×, 6.8×, and 2.6× more males than unbaited control traps. In follow‐up experiments, traps baited with (Z,E)‐α‐farnesene captured significantly more males, but significantly fewer females, than unbaited control traps, indicating sex pheromone autodetection by females. (E)‐β‐Farnesene on its own was not attractive as a trap lure, and when added to (Z,E)‐α‐farnesene at a 20:1 ratio, it reduced the lure’s attractiveness. Our data support the conclusion that (Z,E)‐α‐farnesene is the major sex pheromone component of S. a. destructor females, with both an intra‐ and inter‐sexual communication function.

Vibrational calling signals improve the efficacy of pheromone traps to capture the brown marmorated stink bug

Abstract: Abstract Halyomorpha halys (Stål, 1855), the brown marmorated stink bug (BMSB), is an invasive species that has become a key agricultural pest in its invaded range. Commercial traps available for BMSB monitoring rely on male produced aggregation pheromones as lure, with two possible shortcomings: trap spillover and low detection precision. In this study, we assessed if vibrational signals can increase the attractiveness of pheromone traps by testing the optimized vibration-based lure (Female Song 2, FS2) associated with a specifically designed trap (i.e., the vibrotrap). We evaluated the efficacy of this bimodal trap (i.e., pheromones + vibrations) on females, males and nymphs in controlled conditions (greenhouse) and in the field, in two sites at the margin of two commercial vineyards. In the field, bimodal vibrotraps were compared to three unimodal (i.e., only pheromone) trap types. Both experiments showed that the vibrotrap is highly attractive for BMSB, and the optimized FS2 signal significantly improved its effectiveness. Even though FS2 was selected to target males, the number of trapped females increased as well. Overall, the presented findings show a feasible improvement to future commercial BMSB traps through the synergic use of semiophysicals and semiochemicals. Further research is needed to evaluate the effectiveness of vibrotraps for both early detection and mass trapping.

Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

Abstract: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management.In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant.Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.

Sex Pheromone of the Click Beetle Agriotes pilosellus (Schönherr, 1718)

Abstract: Agriotes pilosellus is a fairly common click beetle species distributed in open deciduous and mixed forests throughout a large area in Europe. To identify its sex pheromone, gland extracts of female beetles were analyzed using gas chromatography-mass spectrometry (GC-MS). The only volatile compounds present in the extracts were geranyl butanoate and (E)-8-hydroxygeranyl dibutanoate in a 1:3 ratio, identified by comparison with synthetic samples. Field experiments revealed a clear attraction of A. pilosellus - males towards traps baited with geranyl butanoate, which could be synergistically enhanced by the factor of almost ten by addition of (E)-8-hydroxygeranyl dibutanoate. The latter compound alone did not show any attractive effect. Both compounds correspond well to the structures known from other Agriotes species and may serve as an effective monitoring tool for entomofaunistic research.

Factors Influencing the Efficacy of Novel Attract-and-Kill (ACTTRA SWD) Formulations Against Drosophila suzukii

Abstract: Abstract In the continental United States, the invasive spotted-wing drosophila (SWD), Drosophila suzukii Matsumura, has become a primary pest of multiple stone and soft-skinned fruits. A new innovative adjuvant formulation, ACTTRA SWD, mixed with a suitable insecticide, constitutes a novel attract-and-kill tactic to manage D. suzukii in fruit crops. We hypothesized that background odors present in crop fields, particularly odors from host fruits, negatively affect the effectiveness of this attract-and-kill formulation, as odors from these sources can compete for insect attraction. Additionally, we evaluated the influence of adult D. suzukii sex and physiological status (age and mating status), and fruit ripeness on its response to the ACTTRA SWD formulation. For this, we used two-choice bioassays to test the response of adult D. suzukii to three ACTTRA SWD formulations (named OR1, TD, and HOOK SWD) in the presence and absence of host fruits (blueberries, raspberries, blackberries, and strawberries). Odors from raspberries were significantly more attractive than those from the TD formulation mixed with spinosad (Entrust). For the HOOK SWD formulation and OR1+Entrust formulation, odors from all the fruit types tested were significantly more attractive than the adjuvants. Compared with females, male D. suzukii were more attracted to the TD formulation over the blueberry fruits. Additionally, age and female mating status but not fruit ripeness influenced D. suzukii attraction to both OR1 and TD formulations. The results from this study indicate that D. suzukii physiological status and host fruit availability impact the efficacy of new attract-and-kill adjuvants such as ACTTRA SWD.

Biosynthesis of iridoid sex pheromones in aphids

Abstract: Iridoid monoterpenes, widely distributed in plants and insects, have many ecological functions. While the biosynthesis of iridoids has been extensively studied in plants, little is known about how insects synthesize these natural products. Here, we elucidated the biosynthesis of the iridoids cis-trans-nepetalactol and cis-trans-nepetalactone in the pea aphid Acyrthosiphon pisum [Harris], where they act as sex pheromones. The exclusive production of iridoids in hind legs of sexual female aphids allowed us to identify iridoid genes by searching for genes specifically expressed in this tissue. Biochemical characterization of candidate enzymes revealed that the iridoid pathway in aphids proceeds through the same sequence of intermediates as described for plants. The six identified aphid enzymes are unrelated to their counterparts in plants, conclusively demonstrating an independent evolution of the entire iridoid pathway in plants and insects. In contrast to the plant pathway, at least three of the aphid iridoid enzymes are likely membrane-bound. We demonstrated that a lipid environment facilitates the cyclization of a reactive enol intermediate to the iridoid cyclopentanoid-pyran scaffold in vitro, suggesting that membranes are an essential component of the aphid iridoid pathway. Altogether, our discovery of this complex insect metabolic pathway establishes the genetic and biochemical basis for the formation of iridoid sex pheromones in aphids and this discovery also serves as a foundation for understanding the convergent evolution of complex metabolic pathways between kingdoms. Significance Statement Plants, animals and microbes produce a plethora of natural products that are important for defense and communication. Most of these compounds show a phylogenetically restricted occurrence, but in rare instances, the same natural product is biosynthesized by organisms in two different kingdoms. The monoterpene-derived iridoids, for example, have been found in more than 50 plant families, but are also observed in several insect orders. The aphid iridoid pathway discovered in this study, one of the longest and most chemically complex insect-derived natural product biosynthetic pathways reported to date, is compared with iridoid biosynthetic pathways in plants and highlights the mechanisms underlying the convergent evolution of metabolic enzymes in insects and plants.

OUP accepted manuscript

Abstract: Aggressive bark beetles colonize and kill healthy conifers through pheromone-mediated mass aggregation. In several species, the focal point of aggregation moves progressively from mass-attacked 'focus trees' to adjacent, unattacked 'recipient trees', resulting in infestation growth. This process, termed 'switching', is hypothesized to be mediated in whole or in part by antiaggregation pheromones released by beetles as colonization intensifies on a focus tree. We tested this hypothesis with the southern pine beetle, Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae: Scolytinae), by using a windvane apparatus that maintained an unbaited, black, cylindrical trap (surrogate for a recipient tree) continuously 4 m downwind from an identical trap baited with synthetic aggregation attractant (surrogate for a focus tree). In two of three replicated trials, addition of pheromone components with antiaggregation activity, endo-brevicomin or verbenone, to the upwind trap caused a significant but small (<15%) increase in the proportion of beetles caught in the downwind trap. In one of two trials with endo-brevicomin, this shift was associated with a significant reduction in catches in the downwind trap and an overall reduction in catches of beetles by the trap pair. This suggests that an inhibitor-induced increase in landings on the recipient relative to the focus tree may be countered by the radial effects of the inhibitor, which at sufficiently high release may reduce responses to both the focus and recipient tree. We discuss spatial factors that might govern the role of antiaggregation pheromones in stimulating infestation growth as well as additional factors that likely govern switching behavior.

Insect pest management with sex pheromone precursors from engineered oilseed plants

Abstract: Abstract Pheromones have become an environmentally friendly alternative to conventional insecticides for pest control. Most current pheromone-based pest control products target lepidopteran pests of high-value crops, as today’s manufacturing processes cannot yet produce pheromones at low enough costs to enable their use for lower-value crops, especially commodity crops. Camelina sativa seeds genetically modified to express ( Z )-11-hexadecenoic acid, a sex pheromone precursor of several moth species, provided the oil from which the precursor was isolated, purified and transformed into the final pheromone. Trap lures containing this pheromone were then assessed for their capacity to manage moth pests in the field. Plant-derived pheromone lures proved equally effective as synthetic pheromone lures in monitoring the diamondback moth, Plutella xylostella , in cabbage and disrupting mating of cotton bollworm, Helicoverpa armigera , in common bean fields. Our study demonstrates the biological efficacy and economic feasibility of pheromone production in plant factories by metabolic engineering of an oilseed crop.

Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

Abstract: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management.In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant.Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.

A Symmetrical Diester as the Sex Attractant Pheromone of the North American Click Beetle Parallelostethus attenuatus (Say) (Coleoptera: Elateridae)

Abstract: Hexanoic acid, 1-octanol, 1,8-octanediol, octyl hexanoate, 1,8-octanediol monohexanoate, and 1,8-octanediol dihexanoate were identified in headspace volatiles collected from the crushed abdomen of a female click beetle of the species Parallelostethus attenuatus (Say) (Elaterinae, tribe Elaterini). In field trials carried out in Illinois, South Carolina, North Carolina, and Virginia, adult male beetles were strongly attracted to 1,8-octanediol dihexanoate alone. Blends of the dihexanoate with one or more of the other compounds proved to be less attractive than the dihexanoate alone, suggesting that the pheromone of this species may consist of a single compound. The symmetrical diester structure of the pheromone is a novel natural product and appears to be structurally unique among insect pheromones.

Ligand‐binding specificities of four odorant‐binding proteins in <i>Conogethes punctiferalis</i>

Abstract: Odorant-binding proteins (OBPs) play essential roles in lepidopteran insects' perception of host volatiles by binding and transporting hydrophobic ligands. The yellow peach moth (YPM), Conogethes punctiferalis (Guenée), is a serious agricultural pest, with broad host range and cryptic feeding habits. However, few studies about YPM perceiving pheromones and host plant odorants have been reported. In this study, four OBP genes (CpunOBP8, CpunOBP9, CpunABP, and CpunGOBP2) were cloned from the antennae of YPM. The recombinant proteins were expressed and purified by prokaryotic expression system, with their binding affinities to 26 ligands being tested. Four CpunOBPs all had six conserved cysteine residues, which were typical structural characteristics of classical OBPs. The fluorescence competitive binding assay indicated that CpunOBP8 and CpunABP could not only exhibit high binding affinities to female sex pheromones, but also to host plant odorants. For example, CpunOBP8 bound strongly with cis-10-hexadecenal, hexadecanal, and so forth, whereas CpunABP bound with cis-10-hexadecenal, camphene, and 3-carene. Comparatively, CpunOBP9 and CpunGOBP2 could only bind with host plant odorants, with CpunOBP9 binding strongly to 3-methyl-1-butanol, hexyl acetate, and so forth, while CpunGOBP2 displaying the widest binding spectra and correlating with 3-carene, pentyl acetate, and so forth. The results indicated that on the one hand, each of the four CpunOBPs had its specific binding spectra when binding and transporting olfactory ligands; on the other hand, the same ligand might be bound to more than one CpunOBPs, which would provide information for the potential application of semiochemicals in controlling YPM.

Toward Chemical Ecology of Plant-Parasitic Nematodes: Kairomones, Pheromones, and Other Behaviorally Active Chemical Compounds.

Abstract: An overview of natural chemical compounds involved in plant-parasitic nematode (PPN) behavior is presented and classified following a system accepted by chemoecologists. Kairomonal and other egg-hatching stimulants, as well as attractants for juveniles, are presented. Sex, aggregation, egg-hatching, and putative diapause PPN pheromones are analyzed and grouped into clusters of primers and releasers. The role of over 500 chemical compounds, both organic and inorganic, involved in PPN behavior is reviewed, with the most widely analyzed and least studied fields of PPN chemical ecology indicated. Knowledge on PPN chemical ecology facilitates environmentally friendly integrated pest management. This could be achieved by disrupting biointeractions between nematodes and their host plants and/or between nematodes. Data on biologically active chemicals reveals targets for resistant plant selection, including through application of gene silencing techniques.

Identification and Expression Profile of Chemosensory Receptor Genes in Aromia bungii (Faldermann) Antennal Transcriptome

Abstract: Simple Summary There are many chemosensory receptor genes involved in insect chemodetection, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs). In contrast to the well-studied Lepidoptera chemosensory receptor genes, the molecular mechanisms of olfactory sensing in Coleoptera are much less understood. The olfactory system plays a crucial role in insect survival. Understanding the olfactory mechanism of insects in depth might provide theoretical guidance for the development of effective pest control measures. The red-necked longicorn beetle, Aromia bungii (Faldermann) (Coleoptera: Cerambycidae), is a wood-boring pest. In order to increase our understanding of the chemosensory receptor genes of the beetle, we first analyzed the transcriptome data of adult A. bungii antennae using bioinformatics, followed by the screening and identification of chemosensory receptor genes. Then, the expression of the chemosensory receptor genes of both male and female adults was examined using qRT-PCR. These findings will provide valuable information for the analysis of the role of chemosensory receptor genes in A. bungii. Abstract The red-necked longicorn beetle, Aromia bungii (Faldermann) (Coleoptera: Cerambycidae), is a major destructive, wood-boring pest, which is widespread throughout the world. The sex pheromone of A. bungii was reported earlier; however, the chemosensory mechanism of the beetle remains almost unknown. In this study, 45 AbunORs, 6 AbunGRs and 2 AbunIRs were identified among 42,197 unigenes derived from the antennal transcriptome bioinformatic analysis of A. bungii adults. The sequence of putative Orco (AbunOR25) found in this study is highly conserved with the known Orcos from other Coleoptera species, and these Orco genes might be potentially used as target genes for the future development of novel and effective control strategies. Tissue expression analysis showed that 29 AbunOR genes were highly expressed in antennae, especially in the antennae of females, which was consistent with the idea that females might express more pheromone receptors for sensing pheromones, especially the sex pheromones produced by males. AbunOR5, 29, 31 and 37 were clustered with the pheromone receptors of the cerambycid Megacyllene caryae, suggesting that they might be putative pheromone receptors of A. bungii. All six AbunGRs were highly expressed in the mouthparts, indicating that these GRs may be involved in the taste perception process. Both AbunIRs were shown to be female-mouthparts-biased, suggesting that they might also be related to the tasting processes. Our study provides some basic information towards a deeper understanding of the chemosensing mechanism of A. bungii at a molecular level.

OUP accepted manuscript

Abstract: Abstract Insect general odorant-binding proteins (GOBPs) play irreplaceable roles in filtering, binding, and transporting host odorants to olfactory receptors. Grapholita funebrana (Treitscheke) (Lepidoptera: Tortricidae), an economically important pest of fruit crops, uses fruit volatiles as cues to locate host plants. However, the functions of GOBPs in G. funebrana are still unknown. Three GOBP genes, namely, GfunGOBP1, GfunGOBP2, and GfunGOBP3, were cloned, and their expression profiles in different tissues were detected by the method of real-time quantitative PCR (RT-qPCR). The binding properties of recombinant GfunGOBPs (rGfunGOBPs) to various ligands were investigated via fluorescence binding assays. The three GfunGOBPs were mainly expressed in the antennae of both male and female moths. All these three rGfunGOBPs could bind to sex pheromones, while having varying affinities toward these pheromones. The three rGfunGOBPs also displayed a wide range of ligand-binding spectrums with tested host odorants. The rGfunGOBP1, rGfunGOBP2, and rGfunGOBP3 bound to 34, 33, and 30 out of the 41 tested odorants, respectively. Three rGfunGOBPs had overlapping binding activities to β-myrcene, (-)-α-phellandrene, and ethyl isovalerate with the Ki less than 3.0 μM. The rGfunGOBP1 and rGfunGOBP3 could selectively bind to several insecticides, whereas rGfunGOBP2 could not. Three rGfunGOBPs had the dual functions of selectively binding to sex pheromones and host odorants. Moreover, the rGfunGOBP1 and rGfunGOBP3 can also serve as ‘signal proteins’ and bind to different insecticides. This study contributed to elucidating the potential molecular mechanism of the olfaction for G. funebrana, and thereby promotes the development of effective botanical attractants or pheromone synergists to control G. funebrana.

Two Odorant-Binding Proteins Involved in the Recognition of Sex Pheromones in Spodoptera litura Larvae.

Abstract: Usually, the recognition of sex pheromone signals is restricted to adult moths. Here, our behavioral assay showed that fourth-instar Spodoptera litura larvae are attracted to cabbage laced with minor sex pheromones Z9,E12-tetradecadienyl acetate (Z9,E12-14:Ac) or Z9-tetradecenyl acetate (Z9-14:Ac). Seven odorant-binding proteins (OBPs) were upregulated after exposure to Z9,E12-14:Ac, and one OBP was upregulated after exposure to Z9-14:Ac. Fluorescence competitive binding assays showed that GOBP2 and OBP7 bound to sex pheromones. RNAi treatment significantly downregulated GOBP2 and OBP7 mRNA expression by 70.37 and 63.27%, respectively. The siOBP-treated larvae were not attracted to Z9,E12-14:Ac or Z9-14:Ac, and the corresponding preference indices were significantly lower than those in siGFP-treated larvae. Therefore, we concluded that GOBP2 and OBP7 are involved in the attraction of S. litura larvae to food containing Z9,E12-14:Ac and Z9-14:Ac. These results provide an important basis for exploring the olfactory mechanisms underlying sex pheromone attraction in moth larvae.

The roles of several sensory neurons and the feedback from egg laying in regulating the germline response to a sex pheromone in C. elegans hermaphrodites

Abstract: Animals broadcast small molecule pheromones that can alter behavior and physiology in conspecifics. Neuronal circuits that regulate these processes remain largely unknown. In C. elegans, male-enriched ascaroside sex pheromone ascr#10, in addition to behavioral effects, expands the population of germline precursor cells in hermaphrodites. Previously, we identified several sensory neurons required for this effect. We also found that feedback from egg laying acts via serotonergic signaling to license the pheromone response in reproducing adults. Here, using newly available reagents, we confirm and extend several of our previous conclusions: a) the ADL neurons are essential for the ascr#10 response, b) phasmid neurons (PHA and PHB) are unlikely to be involved in the ascr#10 response, c) the mod-1 receptor is the main conduit of the serotonergic feedback from egg laying, and d) serotonin remains the only currently known signal of this feedback. Our findings better define the neuronal circuits that mediate the germline response to the major male pheromone.

Structural Insights into the Ligand-Binding and -Releasing Mechanism of Helicoverpa armigera Pheromone-Binding Protein PBP1

Abstract: Cotton bollworm (Helicoverpa armigera) is a worldwide agricultural pest in which the transport of pheromones is indispensable and perceived by pheromone-binding proteins (PBPs). However, three-dimensional structure, pheromone binding, and releasing mechanisms of PBPs are not completely illustrated. Here, we solved three structures of the cotton bollworm HarmPBP1 at different pH values and its complex with ligand, Z-9-hexadecenal. Although apo-HarmPBP1 adopts a common PBP scaffold of six α-helices surrounding a predominantly hydrophobic central pocket, the conformation is greatly distinct from other apo-PBPs. The Z-9-hexadecenal is bound mainly by hydrophobic interaction. The pheromone can enter this cavity through an opening between the helices α5 and α6, as well as the loop between α3 and α4. Structural analysis suggests that ligand entry into the pocket is followed by a shift of Lys94 and Lys138, which may act as a lid at the opening of the pocket. Acidic pH will cause a subtle structural change of the lid, which in turn affects its ligand-binding ability, differently from other family proteins. Taken together, this study provides structural bases for the interactions between pheromones and PBPs, the pH-induced conformational switch, and the design of small inhibitors to control cotton bollworms by disrupting male–female chemosensory communication.

Environmentally responsive reproduction: neuroendocrine signalling and the evolution of eusociality

Abstract: Eusociality is a rare but successful life-history strategy that is defined by the reproductive division of labour. In eusocial species, most females forgo their own reproduction to support that of a dominant female or queen. In many eusocial insects, worker reproduction is inhibited via dominance hierarchies or by pheromones produced by the queen and her brood. Here, we consider whether these cues may act as generic 'environmental signals', similar to temperature or nutrition stress, which induce a state of reproductive dormancy in some solitary insects. We review the recent findings regarding the mechanisms of reproductive dormancy in insects and highlight key gaps in our understanding of how environmental cues inhibit reproduction.

Identification of Cuticular and Web Lipids of the Spider Argiope bruennichi

Abstract: Emerging evidence shows that the cuticular and silk lipids of spiders are structurally more diverse than those of insects, although only a relatively low number of species have been investigated so far. As in insects, such lipids might play a role as signals in various contexts. The wasp spider Argiope bruennichi has probably the best investigated chemical communication system within spiders, including the known structure of the female sex pheromone. Recently we showed that kin-recognition in A. bruennichi could be mediated through the cuticular compounds consisting of hydrocarbons and, to a much larger proportion, of wax esters. By use of mass spectrometry and various derivatization methods, these were identified as esters of 2,4-dimethylalkanoic acids and 1-alkanols of varying chain lengths, such as tetradecyl 2,4-dimethylheptadecanoate. A representative enantioselective synthesis of this compound was performed which proved the identifications and allowed us to postulate that the natural enantiomer likely has the (2R,4R)-configuration. Chemical profiles of the silk and cuticular lipids of females were similar, while male cuticular profiles differed from those of females. Major components of the male cuticular lipids were tridecyl 2,4-dimethyl-C17-19 alkanoates, whereas those of females were slightly longer, comprising tridecyl 2,4-dimethyl-C19-21 alkanoates. In addition, minor female-specific 4-methylalkyl esters were detected.

No evidence for long-range male sex pheromones in two malaria mosquitoes

Abstract: Cues involved in mate seeking and recognition prevent hybridization and can be involved in speciation processes. In malaria mosquitoes, females of the two sibling species Anopheles gambiae s.s. and An. coluzzii mate in monospecific male swarms and hybrids are rare. Long-range sex pheromones driving this behaviour have been debated in literature but so far, no study has proven their existence or their absence. Here, we attempted to bring to light their existence. To put all the odds in our favour, we used different chemical ecology methods such as behavioural and electrophysiological assays as well chemical analyses, and we worked with mosquitoes at their optimal physiological mating state that is with swarming males during their natural swarming windows. Despite all our efforts, our results support the absence of long-range sex pheromones involved in swarm detection and recognition by females. We briefly discuss the implications of this finding in ecology, evolution and for control strategies.