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A. Mark Cigan
Researcher at DuPont Pioneer
Publications - 28
Citations - 3576
A. Mark Cigan is an academic researcher from DuPont Pioneer. The author has contributed to research in topics: Gene & Cas9. The author has an hindex of 19, co-authored 25 publications receiving 2793 citations. Previous affiliations of A. Mark Cigan include DuPont.
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The heterochronic maize mutant Corngrass1 results from overexpression of a tandem microRNA.
TL;DR: It is found that the dominant Corngrass1 mutant of maize encodes two tandem miR156 genes that are overexpressed in the meristem and lateral organs, providing a mechanism for how species-level heterochronic changes can occur in nature.
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Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
TL;DR: Biolistic delivery of pre-assembled Cas9–gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles are reported, opening new opportunities to accelerate breeding practices in a wide variety of crop species.
Journal ArticleDOI
Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA
TL;DR: The examples reported in this study demonstrate the utility of Cas9-guide RNA technology as a plant genome editing tool to enhance plant breeding and crop research needed to meet growing agriculture demands of the future.
Journal ArticleDOI
Cas9-Guide RNA Directed Genome Editing in Soybean
Zhongsen Li,Zhan-Bin Liu,Aiqiu Xing,Bryan P. Moon,Jessica P. Koellhoffer,Lingxia Huang,R. Timothy Ward,Elizabeth Clifton,S. Carl Falco,A. Mark Cigan +9 more
TL;DR: High-frequency and heritable targeted mutagenesis, transgene integration, and endogenous gene editing in soybean is realized using CRISPR Cas9-gRNA.
Journal ArticleDOI
Mapping the genomic landscape of CRISPR–Cas9 cleavage
Cameron Peter Sean,Chris R. Fuller,Paul Daniel Donohoue,Brittnee N. Jones,Matthew S. Thompson,Matthew Merrill Carter,Scott Gradia,Bastien Vidal,Elizabeth Garner,Euan M. Slorach,Elaine Lau,Lynda M. Banh,Alexandra M. Lied,Leslie S. Edwards,Alexander H. Settle,Daniel Capurso,Victor Llaca,Stéphane Deschamps,A. Mark Cigan,Joshua K. Young,Andrew May +20 more
TL;DR: This work developed a biochemical method (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of cut sites within genomic DNA, and found that the number of sites identified depended on sgRNA sequence and nuclease concentration.