M
Matthew S. Thompson
Publications - 5
Citations - 690
Matthew S. Thompson is an academic researcher. The author has contributed to research in topics: Cas9 & Chemistry. The author has an hindex of 3, co-authored 3 publications receiving 503 citations.
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Journal ArticleDOI
DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks
Megan van Overbeek,Daniel Capurso,Matthew Merrill Carter,Matthew S. Thompson,Elizabeth Frias,Carsten Russ,John S. Reece-Hoyes,Christopher D. Nye,Scott Gradia,Bastien Vidal,Jiashun Zheng,Gregory R. Hoffman,Chris R. Fuller,Andrew May +13 more
TL;DR: This work demonstrates that the pattern of DNA repair following Cas9 cutting at each site is nonrandom and consistent across experimental replicates, cell lines, and reagent delivery methods, and elucidates a strategy for using "error-prone" DNA-repair machinery to generate precise edits.
Journal ArticleDOI
Mapping the genomic landscape of CRISPR–Cas9 cleavage
Cameron Peter Sean,Chris R. Fuller,Paul Daniel Donohoue,Brittnee N. Jones,Matthew S. Thompson,Matthew Merrill Carter,Scott Gradia,Bastien Vidal,Elizabeth Garner,Euan M. Slorach,Elaine Lau,Lynda M. Banh,Alexandra M. Lied,Leslie S. Edwards,Alexander H. Settle,Daniel Capurso,Victor Llaca,Stéphane Deschamps,A. Mark Cigan,Joshua K. Young,Andrew May +20 more
TL;DR: This work developed a biochemical method (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of cut sites within genomic DNA, and found that the number of sites identified depended on sgRNA sequence and nuclease concentration.
Journal ArticleDOI
SITE-Seq: A Genome-wide Method to Measure Cas9 Cleavage
Journal ArticleDOI
Adding Polypeptides to the Toolbox for Redox-Switchable Polymerization and Copolymerization Catalysis
Matthew S. Thompson,Stephanie A. Johnson,Stella A. Gonsales,Samantha L. Kristufek,Jeffery A. Byers +4 more
TL;DR: In this article , the authors showed that the reactivity of (2,6MeBIP)FeOCH2C(CH3)3 with Sar-NCA was combined with its known reactivity for ε-caprolactone polymerization to form diblock and triblock copoly(ester-b-peptides) through sequential addition of lactone and NCA monomers.
Journal ArticleDOI
Oligonucleotide mapping via mass spectrometry to enable comprehensive primary structure characterization of an mRNA vaccine against SARS-CoV-2
Brian Gau,Andrew William Dawdy,Hanliu Leah Wang,Bradley Bare,Carlos H. Castaneda,Olga V. Friese,Matthew S. Thompson,Thomas F. Lerch,David Cirelli,Jason C. Rouse +9 more
TL;DR: Oligonucleotide mapping via liquid chromatography with UV detection coupled to tandem mass spectrometry (LC-UV-MS/MS) was recently developed to support development of Comirnaty, the world's first commercial mRNA vaccine which immunizes against the SARS-CoV-2 virus as discussed by the authors .