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Alexander H. Settle
Researcher at Memorial Sloan Kettering Cancer Center
Publications - 4
Citations - 415
Alexander H. Settle is an academic researcher from Memorial Sloan Kettering Cancer Center. The author has contributed to research in topics: Cas9 & CRISPR. The author has an hindex of 3, co-authored 3 publications receiving 292 citations.
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Journal ArticleDOI
Mapping the genomic landscape of CRISPR–Cas9 cleavage
Cameron Peter Sean,Chris R. Fuller,Paul Daniel Donohoue,Brittnee N. Jones,Matthew S. Thompson,Matthew Merrill Carter,Scott Gradia,Bastien Vidal,Elizabeth Garner,Euan M. Slorach,Elaine Lau,Lynda M. Banh,Alexandra M. Lied,Leslie S. Edwards,Alexander H. Settle,Daniel Capurso,Victor Llaca,Stéphane Deschamps,A. Mark Cigan,Joshua K. Young,Andrew May +20 more
TL;DR: This work developed a biochemical method (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of cut sites within genomic DNA, and found that the number of sites identified depended on sgRNA sequence and nuclease concentration.
Journal ArticleDOI
NmeCas9 is an intrinsically high-fidelity genome-editing platform
Nadia Amrani,Xin D. Gao,Pengpeng Liu,Alireza Edraki,Aamir Mir,Raed Ibraheim,Ankit Gupta,Kanae E. Sasaki,Kanae E. Sasaki,Tong Wu,Paul Daniel Donohoue,Alexander H. Settle,Alexandra M. Lied,Kyle McGovern,Chris R. Fuller,Peter Cameron,Thomas G. Fazzio,Lihua Julie Zhu,Scot A. Wolfe,Erik J. Sontheimer +19 more
TL;DR: The results show that NmeCas9 is a naturally high-fidelity genome-editing enzyme and suggest that additional Cas9 orthologs may prove to exhibit similarly high accuracy, even without extensive engineering.
Journal ArticleDOI
SITE-Seq: A Genome-wide Method to Measure Cas9 Cleavage
Posted ContentDOI
Topographical analysis of immune cell interactions reveals a biomechanical signature for immune cytolysis
Miguel M. de Jesus,Alexander H. Settle,Daan Vorselen,Thomas Gaetjens,Michael Galiano,Yung-Yu Wong,Endi K. Santosa,Benjamin Y. Winer,Fella Tamzalit,Mitchell S. Wang,Zhirong Bao,Joseph C. Sun,Pavak K. Shah,Julie A. Theriot,Steven M. Abel,Morgan Huse +15 more
TL;DR: In this article , the authors used super-resolution traction force microscopy to compare cytotoxic T cell immune synapses with contacts formed by other T cell subsets and macrophages.