Joshua K. Young

TL;DR: This work developed a biochemical method (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of cut sites within genomic DNA, and found that the number of sites identified depended on sgRNA sequence and nuclease concentration.

TL;DR: These findings show that miniature Cas12f nucleases can protect against invading dsDNA like much larger class 2 CRISPR effectors and have the potential to be harnessed as programmable nucleases for genome editing.

TL;DR: In this article, compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a target sequence in the genome of a plant or plant cell are provided.

TL;DR: Field trials showed that CRISPR-waxy hybrids were agronomically superior to introgressed hybrids, producing on average 5.5 bushels per acre higher yield.

TL;DR: Characterization of a subset of Cas9 orthologs using purified components reveals additional biochemical diversity, including both narrow and broad ranges of temperature dependence, staggered-end DNA target cleavage, and a requirement for long stretches of homology between gRNA and DNA target.