Alain Laederach

TL;DR: A novel method of analyzing chemical shift perturbation and cross-peak volumes to measure the affinities of both ligands for each SH2 conformer is developed and finds that the cis imide bond containing SH2conformer exhibits a 3.5-fold higher affinity for the Itk SH3 domain compared with binding of the trans conformer to the same ligand.

TL;DR: Single Molecule Cluster Analysis (SiMCAn), which utilizes hierarchical clustering of hidden Markov modeling–fitted single-molecule fluorescence resonance energy transfer (smFRET) trajectories to dissect the complex conformational dynamics of biomolecular machines, enables rapid interpretation of complex single- Molecule behaviors and should prove useful for the comprehensive analysis of a plethora of dynamic cellular machines.

TL;DR: Docking studies were carried out with both open and closed configurations of the β‐amylase mobile flap, a loop comprising residues 96 to 103, suggesting that it is only upon cleavage of the α‐1,4 linkage that optimal closed‐flap binding can occur with the crytallographically determined enzyme structure.

TL;DR: In this paper, the water adsorption isotherm of type IV, observed for a number of carbons near room temperature, can be decomposed into two contributions of types I and V. The corresponding isotherms can be treated as Dubinin-Astakhov equations.

TL;DR: A standard for reporting the results of single nucleotide resolution nucleic acid structure mapping experiments, or SNRNASMs, is developed and a schema for sharing nucleic acids chemical probing data is proposed that uses generic public servers for storing, retrieving, and searching the data.