Alessandra Pasut

TL;DR: It is concluded that dystrophin has an essential role in the regulation of satellite cell polarity and asymmetric division, and muscle wasting in DMD not only is caused by myofiber fragility, but also is exacerbated by impaired regeneration owing to intrinsic satellite cell dysfunction.

TL;DR: It is reported that adult satellite cells give rise to brown adipocytes and that microRNA-133 regulates the choice between myogenic and brown adipose determination by targeting the 3'UTR of Prdm16 and it is demonstrated that miR-133 levels are downregulated in mice exposed to cold, resulting in de novo generation of satellite cell-derivedbrown adipocytes.

TL;DR: It is reported that EZH2, the enzymatic subunit of the Polycomb-repressive complex 2 (PRC2), is expressed in both Pax7+/Myf5⁻ stem cells and Pax7/My f5+ committed myogenic precursors and is required for homeostasis of the adult SC pool.

TL;DR: The protocol currently used in the laboratory to isolate single myofibers from the Extensor Digitorum Longus (EDL) muscle of adult mice (6-8 weeks old) is described.

TL;DR: Functional assay shows that Integrin α7(+)/CD34(+) double positive cells and CD45, CD31, CD11b, and Sca1 negative (Lin(-)) cells possess high myogenic potential and ability to regenerate muscle depleted satellite cells upon transplantation.