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Amanda Nourse
Researcher at St. Jude Children's Research Hospital
Publications - 49
Citations - 3973
Amanda Nourse is an academic researcher from St. Jude Children's Research Hospital. The author has contributed to research in topics: Ubiquitin ligase & Protein structure. The author has an hindex of 27, co-authored 46 publications receiving 3060 citations. Previous affiliations of Amanda Nourse include University of Queensland.
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Journal ArticleDOI
Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Min Zhuang,Matthew F. Calabrese,Jiang Liu,M. Brett Waddell,Amanda Nourse,Michal Hammel,Darcie J. Miller,Helen Walden,David M. Duda,Steven N. Seyedin,Timothy Hoggard,Timothy Hoggard,J. Wade Harper,Kevin P. White,Brenda A. Schulman,Brenda A. Schulman +15 more
TL;DR: In this paper, the authors define a SPOP-binding consensus (SBC) and determine structures revealing recognition of SBCs from the phosphatase Puc, the transcriptional regulator Ci, and the chromatin component MacroH2A.
Journal ArticleDOI
Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA
Diana M. Mitrea,Jaclyn A Cika,Jaclyn A Cika,Clifford S. Guy,David Ban,Priya R. Banerjee,Christopher B. Stanley,Amanda Nourse,Ashok A. Deniz,Richard W. Kriwacki,Richard W. Kriwacki +10 more
TL;DR: It is shown that nucleophosmin (NPM1) integrates within the nucleolus via a multi-modal mechanism involving multivalent interactions with proteins containing arginine-rich linear motifs (R-motifs) and ribosomal RNA (rRNA), which are found in canonical nucleolar localization signals.
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A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing
Ailin Wang,Alexander E. Conicella,Hermann Broder Schmidt,Erik W. Martin,Shannon N Rhoads,Ashley N. Reeb,Amanda Nourse,Daniel Ramirez Montero,Veronica H Ryan,Rajat Rohatgi,Frank Shewmaker,Mandar T. Naik,Tanja Mittag,Yuna M. Ayala,Nicolas L. Fawzi +14 more
TL;DR: The fusion‐tag solubilized, recombinant form of TDP‐43 full‐length protein developed here will enable future phase separation and in vitro biochemical assays on TDP43 function and interactions that have been hampered in the past by T DP‐43 aggregation.
Journal ArticleDOI
Self-interaction of NPM1 modulates multiple mechanisms of liquid–liquid phase separation
Diana M. Mitrea,Jaclyn A Cika,Jaclyn A Cika,Jaclyn A Cika,Christopher B. Stanley,Amanda Nourse,Paulo L. Onuchic,Priya R. Banerjee,Priya R. Banerjee,Aaron H. Phillips,Cheon-Gil Park,Ashok A. Deniz,Richard W. Kriwacki,Richard W. Kriwacki +13 more
TL;DR: It is proposed that this newly discovered interplay between multiple LLPS mechanisms may influence the direction of vectorial pre-ribosomal particle assembly within, and exit from the nucleolus as part of the ribosome biogenesis process.
Journal ArticleDOI
DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome
Parimal Samir,Sannula Kesavardhana,Deanna M. Patmore,Sebastien Gingras,Sebastien Gingras,R. K. Subbarao Malireddi,Rajendra Karki,Clifford S. Guy,Benoit Briard,David E. Place,Anannya Bhattacharya,Bhesh Raj Sharma,Amanda Nourse,Sharon V. King,Aaron Pitre,Amanda R. Burton,Stephane Pelletier,Richard J. Gilbertson,Thirumala-Devi Kanneganti +18 more
TL;DR: The findings suggest that macrophages use the availability of DDX3X to interpret stress signals and choose between pro-survival stress granules and pyroptotic ASC specks, and suggest a rheostat-like mechanistic paradigm for regulating live-or-die cell-fate decisions under stress conditions.