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Ana Carvalho
Researcher at University of Edinburgh
Publications - 16
Citations - 1276
Ana Carvalho is an academic researcher from University of Edinburgh. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 6, co-authored 10 publications receiving 1199 citations. Previous affiliations of Ana Carvalho include Institute of Molecular Pathology and Immunology of the University of Porto & Ludwig Institute for Cancer Research.
Papers
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Journal ArticleDOI
Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.
Reto Gassmann,Ana Carvalho,Ana Carvalho,Alexander J. Henzing,Sandrine Ruchaud,Damien F. Hudson,Reiko Honda,Erich A. Nigg,Dietlind L. Gerloff,William C. Earnshaw +9 more
TL;DR: Borealin is described and results implicate the chromosomal passenger holocomplex in the maintenance of spindle integrity and suggest that histone H3 serine10 phosphorylation is performed by an Aurora B-INCENP subcomplex.
Journal ArticleDOI
Survivin is required for stable checkpoint activation in taxol-treated HeLa cells.
TL;DR: RNA interference is used to specifically repress Survivin in cultured HeLa cells to reveal that Survivin is not required for the spindle assembly checkpoint when it is activated by the loss of microtubules, but is required forThe maintenance of the checkpointWhen it isactivated by taxol, which is generally thought to cause a loss of spindle tension.
Journal ArticleDOI
INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis.
TL;DR: The data provide the first biochemical evidence that Survivin can interact directly with members of the chromosomal passenger complex and in vitro interaction between Survivin and Aurora-B is extraordinarily stable in that it resists 3 M NaCl.
Journal ArticleDOI
Deconstructing Survivin: comprehensive genetic analysis of Survivin function by conditional knockout in a vertebrate cell line
Zuojun Yue,Ana Carvalho,Zhenjie Xu,Xuemei Yuan,Stefano Cardinale,Susana A. Ribeiro,Fan Lai,Hiromi Ogawa,Elisabet Gudmundsdottir,Reto Gassmann,Ciaran G. Morrison,Sandrine Ruchaud,William C. Earnshaw +12 more
TL;DR: In this article, the authors describe the isolation of two conditional knockouts of the Survivin gene in chicken DT40 cells and show that these cells can grow normally if their sole Survivin is missing a widely studied cyclindependent kinase phosphorylation site or sites reportedly essential for binding to Smac or aurora B.
Book ChapterDOI
Affinity purification of protein complexes in C. elegans.
Esther Zanin,Julien Dumont,Reto Gassmann,Iain M. Cheeseman,Paul S. Maddox,Shirin Bahmanyar,Ana Carvalho,Sherry Niessen,John R. Yates,Karen Oegema,Arshad Desai +10 more
TL;DR: In this paper, the authors focus on two strategies to identify protein-protein interactions: single-step immunoprecipitation and tandem affinity purification, and describe methods for growth of worms in large-scale liquid culture, preparation of worm and embryo extracts.