Anna Kula-Pacurar

TL;DR: It is shown that fully differentiated human airway epithelium cultures constitute an excellent model to study infection with the novel human coronavirus SARS-CoV-2, and efficient replication of the virus in the tissue, with maximal replication at 2 days postinfection.

TL;DR: In this article, the effect of organoselenium functionalities in the C-8 seleno-quercetin derivatives was explored, and it was shown that a hydrogen bond between the selenium atom and Gln189 residue in the catalytic pocket could explain the higher Mpro activity of 2d and, as a result, its better antiviral profile.

TL;DR: Here, fully differentiated human airway epithelium cultures are proposed as a model for studies on the SARS-CoV-2 and basic characteristics of the system are provided.

TL;DR: The "shock-and-kill" strategy is one of the most explored HIV-1 cure approaches to eliminate latent virus as discussed by the authors, which is based on using latency reversing agents (LRAs) to reactivate latent proviruses and to induce subsequent elimination of the reactivated cells by immune responses or virus-induced cytopathic effects.

TL;DR: In this article, a protocol for in situ hybridization chain reaction (HCR) coupled with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in cell line and three-dimensional (3D) cultures of human airway epithelium.