Anthony J. Garrity

TL;DR: The extent, origins and consequences of genetic variation within human cell lines are studied, providing a framework for researchers to measure such variation in efforts to support maximally reproducible cancer research.

TL;DR: A pooled approach for optical genetic screens in mammalian cells using targeted in situ sequencing to demultiplex a library of genetic perturbations following image-based phenotyping with pooled libraries is introduced.

TL;DR: It is shown that modifying the packaging protocol by diluting the perturbation library with a carrier plasmid increases the fraction of correct, single integrations from <60% to >90%, at the cost of reducing titer by 100-fold.

TL;DR: This work introduces an optical method to link perturbations and their phenotypic outcomes at the singlecell level in a pooled setting and applies this technology to screen a focused set of 952 genes across >3 million cells for involvement in NF-κB activation.

TL;DR: It is demonstrated that CloneSifter can measure clonal fitness of cancer cell models in vitro and retrieve targeted clones at abundance as low as 1 in 1883 in a heterogeneous cell population.