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Atsuo Ogura
Researcher at University of Tsukuba
Publications - 321
Citations - 19720
Atsuo Ogura is an academic researcher from University of Tsukuba. The author has contributed to research in topics: Stem cell & Embryonic stem cell. The author has an hindex of 66, co-authored 305 publications receiving 17834 citations. Previous affiliations of Atsuo Ogura include National Institutes of Health & Kurume University.
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Journal ArticleDOI
Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
Mito Kanatsu-Shinohara,Narumi Ogonuki,Kimiko Inoue,Hiromi Miki,Atsuo Ogura,Shinya Toyokuni,Takashi Shinohara +6 more
TL;DR: In vitro culture of spermatogonial stem cells that proliferate for long periods of time are reported, and gonocytes isolated from neonatal mouse testis proliferated over a 5-month period and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules.
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Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue.
TL;DR: Hydroxy naphthol blue (HNB), a metal indicator for calcium and a colorimetric reagent for alkaline earth metal ions, was used for a newcolorimetric assay of the LAMP reaction, which would be helpful for high-throughput DNA and RNA detection.
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Generation of Pluripotent Stem Cells from Neonatal Mouse Testis
Mito Kanatsu-Shinohara,Kimiko Inoue,Jiyoung Lee,Momoko Yoshimoto,Narumi Ogonuki,Hiromi Miki,Shiro Baba,Takeo Kato,Yasuhiro Kazuki,Shinya Toyokuni,Megumi Toyoshima,Ohtsura Niwa,Mitsuo Oshimura,Toshio Heike,Tatsutoshi Nakahata,Fumitoshi Ishino,Atsuo Ogura,Takashi Shinohara +17 more
TL;DR: The successful establishment of ES-like cells from neonatal mouse testis were phenotypically similar to ES/EG cells except in their genomic imprinting pattern and differentiated into various types of somatic cells in vitro under conditions used to induce the differentiation of ES cells.
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Requirement of CD9 on the egg plasma membrane for fertilization.
Kenji Miyado,G Yamada,Shuichi Yamada,Hidetoshi Hasuwa,Yasuhiro Nakamura,Fuminori Ryu,Kentaro Suzuki,Ken-ichiro Kosai,Kimiko Inoue,Atsuo Ogura,Masaru Okabe,Eisuke Mekada +11 more
TL;DR: In vitro fertilization experiments indicate that CD9 has a crucial role in sperm-egg fusion.
Journal ArticleDOI
In vitro production of functional sperm in cultured neonatal mouse testes
Takuya Sato,Kumiko Katagiri,Ayako Gohbara,Kimiko Inoue,Narumi Ogonuki,Atsuo Ogura,Yoshinobu Kubota,Takehiko Ogawa +7 more
TL;DR: It is shown that neonatal mouse testes which contain only gonocytes or primitive spermatogonia as germ cells can produce sperMatids and sperm in vitro with serum-free culture media and resulted in healthy and reproductively competent offspring through microinsemination.