Austin B. Tomaney

TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.

TL;DR: The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations and is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time.

TL;DR: In this article, the authors describe a sequence-by-incorporation (SBI) method for processing signal data from analytical operations and systems, and particularly in processing data from sequence by incorporation processes to identify nucleotide sequences of template nucleic acids.

TL;DR: In this article, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. But these methods are not suitable for the characterization of nucleic acids.

TL;DR: In this article, the authors define and apply a 2-dimensional software mask for obtaining signal data from arrays with higher signal to noise than where the mask is not applied, and apply it to arrays of sequence-by-incorporation processes to identify nucleotide sequences.