Primo Baybayan

TL;DR: An approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost is reported, effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.

TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.

TL;DR: A high-quality de novo genome assembly from a single Anopheles coluzzii mosquito, using a modified SMRTbell library construction protocol without DNA shearing and size selection, which puts PacBio-based assemblies in reach for small highly heterozygous organisms that comprise much of the diversity of life.

TL;DR: Genomic clades may represent functional units of genetic isolation in Bp, modulating intraspecies genetic diversity, and it is demonstrated that Bp RM systems can inhibit uptake of non-self DNA.

TL;DR: Eight high-coverage SMRT sequence datasets from five organisms that have been publicly released to the general scientific community are described and used to generate whole-genome assemblies, test new algorithms, investigate genome structure and evolution, and identify base modifications in some of the most widely-studied model systems in biological research.