Bernhard Kuster

TL;DR: The results show that phosphorylation site identification is generally possible with false localization rates of about 10%.

TL;DR: A score (OScore) is developed that discriminates O-GlcNAc peptide spectra from spectra of unmodified peptides with 95% sensitivity and >99% specificity and is Integrating the OScore into the two-stage LC-MS/MS approach detected O- Glc NAc peptides in the low fmol range and at 10-fold better sensitivity than a single data-dependent ETD tandem MS experiment.

TL;DR: It is shown that mutants of a DEN1 homolog from Arabidopsis thaliana have no detectable defects in NEDD8 processing but do accumulate a broad range of NEDd8 conjugates, thus providing direct evidence for the existence of non-cullin NedD8 conjUGates.

TL;DR: Recombinant mouse gelatinase B was expressed in the yeast Pichia pastoris and the N-linked oligosaccharides of the truncated glycoprotein variants were analysed by in gel enzymatic release followed by mass spectrometry and normal phase HPLC.

TL;DR: Physical and genetic interactions between Transport Protein Particle II (TRAPPII) tethering factors and microtubule-associated proteins of the PLEIADE/AtMAP65 family are document and provide a conceptual framework for how membrane and micro Tubule dynamics may be coordinated with each other and with the nuclear cycle during plant cytokinesis.