B
Bernhard Kuster
Researcher at Technische Universität München
Publications - 310
Citations - 37543
Bernhard Kuster is an academic researcher from Technische Universität München. The author has contributed to research in topics: Proteome & Proteomics. The author has an hindex of 79, co-authored 277 publications receiving 31872 citations. Previous affiliations of Bernhard Kuster include GlaxoSmithKline & German Cancer Research Center.
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Journal ArticleDOI
Generating high quality libraries for DIA MS with empirically corrected peptide predictions
Brian C. Searle,Kristian E. Swearingen,Chris Barnes,Tobias Schmidt,Siegfried Gessulat,Bernhard Kuster,Mathias Wilhelm +6 more
TL;DR: A library generation workflow that leverages fragmentation and retention time prediction to build libraries containing every peptide in a proteome, and then refines those libraries with empirical data is demonstrated.
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Comprehensive and Reproducible Phosphopeptide Enrichment Using Iron Immobilized Metal Ion Affinity Chromatography (Fe-IMAC) Columns
Benjamin Ruprecht,Heiner Koch,Guillaume Médard,Max Mundt,Bernhard Kuster,Bernhard Kuster,Simone Lemeer,Simone Lemeer +7 more
TL;DR: The results strongly suggest that insufficient capacity, inefficient elution, and the stochastic nature of data-dependent acquisition in mass spectrometry are the causes of the experimentally observed complementarity.
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Carbonyl-Reactive Tandem Mass Tags for the Proteome-Wide Quantification of N-Linked Glycans
Hannes Hahne,Patrick Neubert,Karsten Kuhn,Chris Etienne,Ryan Bomgarden,John C. Rogers,Bernhard Kuster,Bernhard Kuster +7 more
TL;DR: Stable isotope labeled carbonyl-reactive tandem mass tags (glyco-TMTs) are explored as a novel approach for the quantification of N-linked glycans and revealed significant down-regulation of high-mannose glycans in the metastatic cell line.
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Optimized chemical proteomics assay for kinase inhibitor profiling
Guillaume Médard,Fiona Pachl,Benjamin Ruprecht,Susan Klaeger,Susan Klaeger,Stephanie Heinzlmeir,Stephanie Heinzlmeir,Dominic Helm,Huichao Qiao,Xin Ku,Mathias Wilhelm,Thomas Kuehne,Zhixiang Wu,Antje Dittmann,Carsten Hopf,Karl Kramer,Bernhard Kuster,Bernhard Kuster +17 more
TL;DR: The optimization of the kinobead assay is reported resulting in the combination of five chemical probes and four cell lines to cover half the human kinome in a single assay (∼ 260 kinases).
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Glycosylation of natural human neutrophil gelatinase B and neutrophil gelatinase B-associated lipocalin.
Pauline M. Rudd,Taj S. Mattu,S Masure,T Bratt,P. E. Van den Steen,Mark R. Wormald,Bernhard Kuster,David Harvey,N Borregaard,J Van Damme,Raymond A. Dwek,Ghislain Opdenakker +11 more
TL;DR: Although both proteins were isolated from neutrophils and contained O-linked glycans mainly with type 2 cores, the glycans attached to individual serine/threonine residue(s) in NGAL were significantly smaller than those on gelatinase B.