Bryan R. Cullen

TL;DR: Recent publications are discussed that analyze the ability of the bacterial CRISPR/Cas DNA editing machinery to be repurposed as a tool for the specific cleavage and destruction of HBV cccDNAs in the nuclei of infected cells and consider which steps will be necessary to make CRISpr/Cas targeting ofHBV DNA a clinically feasible approach to the treatment of chronic infections in humans.

TL;DR: Primers designed to detect epizootic haematopoietic necrosis virus and a PCR and dot-blot system were used successfully in screening for the presence of BIV nucleic acid in digested formalin-fixed, paraffin-embedded amphibian tissues, and PCR could detect BIV in amphibians long after BIV challenge, and in frogs which appeared healthy.

TL;DR: It is reported that substitution of glycine for valine at residue 5 in CCR-5 can significantly enhance the level of envelope-dependent cell fusion by expressing cells and hypothesize that these changes may inhibit a conformational transition in hCCR-5 that contributes to HIV-1 infection.

TL;DR: This analysis predicts the existence of a nuclear export receptor distinct from transportin that nevertheless shares a common protein-binding site on heterogeneous nuclear ribonucleoprotein A1.

TL;DR: It is shown that a mutant form of human Dicer lacking the amino-terminal helicase domain can process double-stranded RNAs to produce high levels of siRNAs that are readily detectable by Northern blot, are loaded into RNA-induced silencing complexes, and can effectively and specifically inhibit the expression of cognate mRNAs.