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Carla P. Guimaraes
Researcher at Massachusetts Institute of Technology
Publications - 27
Citations - 5838
Carla P. Guimaraes is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Sortase & Sortase A. The author has an hindex of 21, co-authored 27 publications receiving 5168 citations.
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Journal ArticleDOI
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Luke A. Gilbert,Max A. Horlbeck,Britt Adamson,Jacqueline E. Villalta,Yuwen Chen,Evan H. Whitehead,Carla P. Guimaraes,Barbara Panning,Hidde L. Ploegh,Michael C. Bassik,Lei S. Qi,Martin Kampmann,Jonathan S. Weissman +12 more
TL;DR: This work identifies rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90%-99% knockdown with minimal off-target effects, and activators to endogenous genes via endonuclease-deficient Cas9, which enable modulation of gene expression over a ∼1,000-fold range.
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Luke A. Gilbert,Max A. Horlbeck,Britt Adamson,Jacqueline E. Villalta,Yuwen Chen,Evan H. Whitehead,Carla P. Guimaraes,Barbara Panning,Michael C. Bassik,Lei S. Qi,Martin Kampmann,Jonathan S. Weissman,Hidde L. Ploegh +12 more
TL;DR: In this article, the authors identify rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90%-99% knockdown with minimal off-target effects, and activators (CRisPRa) to endogenous genes via endonuclease-deficient Cas9.
Journal ArticleDOI
Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
Jan E. Carette,Carla P. Guimaraes,Malini Varadarajan,Annie S. Park,Irene Wuethrich,Alzbeta Godarova,Maciej Kotecki,Brent H. Cochran,Eric Spooner,Hidde L. Ploegh,Thijn R. Brummelkamp +10 more
TL;DR: Using insertional mutagenesis to develop a screening method to generate null alleles in a human cell line haploid for all chromosomes except chromosome 8, host factors essential for infection with influenza and genes encoding important elements of the biosynthetic pathway of diphthamide, which are required for the cytotoxic effects ofdiphtheria toxin and exotoxin A are identified.
Journal ArticleDOI
Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity
John M. Antos,Guo-Liang Chew,Carla P. Guimaraes,Nicholas C. Yoder,Gijsbert M. Grotenbreg,Maximilian Wei-Lin Popp,Hidde L. Ploegh +6 more
TL;DR: The generality of N-terminal labeling with SrtAstaph is demonstrated by near-quantitative labeling of multiple protein substrates with excellent site specificity.
Journal ArticleDOI
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Carla P. Guimaraes,Martin D. Witte,Christopher S. Theile,Gunes Bozkurt,Lenka Kundrat,Annet E M Blom,Hidde L. Ploegh +6 more
TL;DR: In this paper, the authors describe the expression and purification conditions for two sortase A enzymes that have different recognition sequences and provide a protocol that allows the functionalization of any given protein at its C terminus, or, for select proteins, at an internal site.