C
Charles M. Nicolet
Researcher at University of Wisconsin-Madison
Publications - 21
Citations - 1313
Charles M. Nicolet is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Saccharomyces cerevisiae & Gene. The author has an hindex of 10, co-authored 21 publications receiving 1283 citations. Previous affiliations of Charles M. Nicolet include University of Southern California & Stanford University.
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Journal ArticleDOI
The translation machinery and 70 kd heat shock protein cooperate in protein synthesis
R. John Nelson,Thomas Ziegelhoffer,Charles M. Nicolet,Margaret Werner-Washburne,Elizabeth A. Craig +4 more
TL;DR: It is suggested that cytosolic hsp70 aids in the passage of the nascent polypeptide chain through the ribosome in a manner analogous to the role played by organelle-localized hsp 70 in the transport of proteins across membranes.
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Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae.
TL;DR: Overexpression of the STI1 gene resulted in substantial trans-activation of SSA4 promoter-reporter gene fusions, indicating that STi1 may play a role in mediating the heat shock response of some HSP70 genes.
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SSC1, an essential member of the yeast HSP70 multigene family, encodes a mitochondrial protein.
Elizabeth A. Craig,J Kramer,J Shilling,Margaret Werner-Washburne,S Holmes,J Kosic-Smithers,Charles M. Nicolet +6 more
TL;DR: It is concluded that Ssc1p is a mitochondrial protein and that hsp70 proteins perform functions in many compartments of the cell.
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A yeast excision-repair gene is inducible by DNA damaging agents
TL;DR: Plasmids containing various RAD-lacZ gene fusions were integrated into the chromosome of haploid yeast cells and induction of the RAD2 gene after treatment of untransformed cells with 4-nitroquinoline 1-oxide was confirmed by direct examination of RAD2 mRNA.
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Evaluation of commercially available RNA amplification kits for RNA sequencing using very low input amounts of total RNA
Savita Shanker,Ariel Paulson,Howard J. Edenberg,Allison Peak,Anoja Perera,Yuriy O. Alekseyev,Nicholas Beckloff,Nathan J. Bivens,Robert J. Donnelly,Allison F. Gillaspy,Deborah S. Grove,Weikuan Gu,Nadereh Jafari,Joanna S. Kerley-Hamilton,Robert H. Lyons,Clifford G. Tepper,Charles M. Nicolet +16 more
TL;DR: In this paper, four types of amplification kits were tested with 3 different concentrations, from 5 ng to 50 pg, of a commercially available RNA. But no single kit appeared to meet all the challenges of small input material.