Charlotte M. Fare

TL;DR: Karyopherin-β2 prevents RBPs with PY-NLSs accumulating in stress granules, restores nuclear RBP localization and function, and rescues degeneration caused by disease-linked FUS and hnRNPA2, establishing that NIRs therapeutically restore RBP homeostasis and mitigate neurodegeneration.

TL;DR: It is demonstrated that wild-type FUS binds single-stranded RNA stoichiometrically in a length-dependent manner and that multimers induce highly dynamic interactions with RNA, giving rise to small and fluid condensates.

TL;DR: In this article, a review of the underlying physical and chemical processes that generate internal condensate architectures is presented, and the authors discuss how specific condensat organization is critical for specific biological functions.

TL;DR: It is demonstrated that ALS/FTLD-linked FUS mutations in glycine (G) strikingly drive formation of droplets that do not readily interact withWT FUS, whereas arginine (R) mutants form mixed condensates with WT FUS.

TL;DR: It is shown that arginine-rich DPRs (poly-GR and poly-PR) bind directly to multiple importins and, in excess, promote their insolubility and condensation, and suggest that importins can decrease toxicity of arginin- rich DPRs by suppressing their pathological interactions.