Hye Ran Koh

TL;DR: Three-color alternating-laser excitation (3c-ALEX) is introduced, a fluorescence resonance energy transfer (FRET) method that measures up to three intramolecular distances and complex interaction stoichiometries of single molecules in solution and paves the way for advanced single-molecule analysis of complex mixtures and biomolecular machinery.

TL;DR: It is demonstrated that wild-type FUS binds single-stranded RNA stoichiometrically in a length-dependent manner and that multimers induce highly dynamic interactions with RNA, giving rise to small and fluid condensates.

TL;DR: A single-molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by SRS2 reveals an exquisite and highly specific mechanism by which Srs2 regulates the Rad51 filaments formation.

TL;DR: The results implicate the dsRNA-specific diffusion activity of TRBP that contributes to enhancing siRNA and miRNA processing by Dicer and imply a universality of the diffusion activity among this protein family.

TL;DR: Fluorescence lifetime analysis of a single Cy3 fluorophore attached to the promoter region of the DNA can be used to reveal transient reaction intermediates during transcription initiation by T7 RNA polymerase, the first demonstration of real-time biochemical reactions observed via single molecule fluorescence lifetime trajectories of immobilized molecules.