C
Christiaan van Ooij
Researcher at Francis Crick Institute
Publications - 12
Citations - 446
Christiaan van Ooij is an academic researcher from Francis Crick Institute. The author has contributed to research in topics: Plasmodium falciparum & Plasmodium. The author has an hindex of 7, co-authored 12 publications receiving 327 citations. Previous affiliations of Christiaan van Ooij include University of London.
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Journal ArticleDOI
Generating conditional gene knockouts in Plasmodium - a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9.
TL;DR: Novel CRISPR/Cas9 transfection plasmids and approaches for the speedy, stable and marker-free introduction of transgenes encoding the DiCre recombinase into genomic loci dispensable for blood stage development are described.
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Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress
Victoria L. Hale,Jean M. Watermeyer,Fiona Hackett,Gema Vizcay-Barrena,Christiaan van Ooij,James A. Thomas,Matthew C. Spink,Maria Harkiolaki,Elizabeth Duke,Roland A. Fleck,Michael J. Blackman,Michael J. Blackman,Helen R. Saibil +12 more
TL;DR: This work describes a previously unidentified permeabilization of the vacuolar membrane at the start of egress, preceding membrane rupture, suggesting a new initiation step in egress and shows that host cell cytoskeleton breakdown is restricted to a narrow time window within the final stages of Egress.
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The Plasmodium falciparum rhoptry protein RhopH3 plays essential roles in host cell invasion and nutrient uptake
Emma S Sherling,Emma S Sherling,Ellen Knuepfer,Joseph Brzostowski,Louis H. Miller,Michael J. Blackman,Michael J. Blackman,Christiaan van Ooij +7 more
TL;DR: A dual role for RhopH3 is identified that links erythrocyte invasion to formation of the PSAC/NPP essential for parasite survival within host ERYthrocytes.
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Variant Exported Blood-Stage Proteins Encoded by Plasmodium Multigene Families Are Expressed in Liver Stages Where They Are Exported into the Parasitophorous Vacuole.
Aurélie Fougère,Aurélie Fougère,Andrew P. Jackson,Dafni Paraskevi Bechtsi,Joanna A. M. Braks,Takeshi Annoura,Takeshi Annoura,Jannik Fonager,Roberta Spaccapelo,Jai Ramesar,Severine Chevalley-Maurel,Onny Klop,Onny Klop,Annelies M. A. van der Laan,Hans J. Tanke,Clemens H. M. Kocken,Erica M. Pasini,Shahid M. Khan,Ulrike Böhme,Christiaan van Ooij,Thomas D. Otto,Chris J. Janse,Blandine Franke-Fayard +22 more
TL;DR: This is the first demonstration of a biological function of any exported variant protein family of rodent malaria parasites and indicates that these proteins may transport (host) phosphatidylcholine for membrane synthesis in vitro.
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Host cell remodeling by pathogens: the exomembrane system in Plasmodium-infected erythrocytes
TL;DR: An overview of the different parts of the exomembrane system is provided, describing the parasitophorous vacuole, the tubovesicular network, Maurer's clefts, the caveola-vesicle complex, J dots and other mobile compartments, and the small vesicles that have been observed in Plasmodium-infected cells.