Matthew C. Spink

TL;DR: This work describes a previously unidentified permeabilization of the vacuolar membrane at the start of egress, preceding membrane rupture, suggesting a new initiation step in egress and shows that host cell cytoskeleton breakdown is restricted to a narrow time window within the final stages of Egress.

TL;DR: An overview of the technique, discussion of sample suitability and information about sample preparation, data collection and data analysis is presented, and recent developments and future outlook are also discussed.

TL;DR: A new workbench named SuRVoS (Super-Region Volume Segmentation), within this software, a volume to be segmented is first partitioned into hierarchical segmentation layers and is then interactively segmented with the user's knowledge input in the form of training annotations.

TL;DR: An integrated, user-friendly platform for 3D correlative imaging of cells in vitreous ice by using super-resolution structured illumination microscopy in conjunction with soft X-ray tomography is developed.

TL;DR: In this paper, the authors investigated the possibility of a dynamic exchange between ordered and disordered states during nuclear transport by using electron microscopy of S. cerevisiae and found that the anchor domain of a GLFG nucleoporin locates exclusively to the central channel and could be cytoplasmic, central or nucleoplasmic.