Cuicui Liu

TL;DR: Single-cell RNA sequencing of human MKs from embryonic yolk sac and fetal liver is used to characterize the transcriptome, cellular heterogeneity, and developmental trajectories of early megakaryopoiesis and identifies a subpopulation of CD42b+CD14+ MKs in vivo that exhibit high expression of genes associated with immune responses.

TL;DR: A critical role of muscle segment homeobox 2 (MSX2) is identified in initiating and accelerating the molecular program that leads to mesenchymal stem/stromal cell (MSC) differentiation from hPSCs.

TL;DR: Myeloid ectopic viral integration site 1 homolog (MEIS1) is identified as a crucial regulator of hPSC early hematopoietic differentiation and can be potentially manipulated for large-scale generation of HPCs or platelets from hPSCs for therapeutic applications in regenerative medicine.

TL;DR: It is found that a rotary cell culture system (RCCS) can potentiate megakaryopoiesis and significantly improve the efficiency of platelet generation, an important step toward large‐scale platelet production for future biomedical and clinical applications.

TL;DR: The results demonstrate that autonomous production of cytokines in hESCs may become a powerful approach for cost-effective and large-scale platelet generation in translational medicine.