D
David N. Bolam
Researcher at Newcastle University
Publications - 74
Citations - 8435
David N. Bolam is an academic researcher from Newcastle University. The author has contributed to research in topics: Carbohydrate-binding module & Glycan. The author has an hindex of 46, co-authored 69 publications receiving 7461 citations. Previous affiliations of David N. Bolam include University of East Anglia & University of Newcastle.
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Journal ArticleDOI
Carbohydrate-binding modules: fine-tuning polysaccharide recognition
TL;DR: The present review summarizes the impact structural biology has had on the understanding of the mechanisms by which CBMs bind to their target ligands.
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Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts.
Eric C. Martens,Eric C. Martens,Elisabeth C. Lowe,Herbert C. Chiang,Nicholas A. Pudlo,Meng-Meng Wu,Nathan P. McNulty,D. Wade Abbott,Bernard Henrissat,Harry J. Gilbert,Harry J. Gilbert,David N. Bolam,Jeffrey I. Gordon +12 more
TL;DR: Competition for nutrients contained in diverse types of plant cell wall-associated polysaccharides may explain the evolution of substrate-specific catabolic gene modules in common bacterial members of the human gut microbiota.
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Specificity of Polysaccharide Use in Intestinal Bacteroides Species Determines Diet-Induced Microbiota Alterations
Erica D. Sonnenburg,Hongjun Zheng,Payal Joglekar,Steven K. Higginbottom,Susan J. Firbank,David N. Bolam,Justin L. Sonnenburg +6 more
TL;DR: The basis of Bacteroides proliferation in response to fructans, a class of fructose-based dietary polysaccharides, is elucidated and gene sequences that distinguish species' metabolic capacity serve as potential biomarkers in microbiomic datasets to enable rational manipulation of the microbiota via diet.
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Glycan complexity dictates microbial resource allocation in the large intestine
Artur Rogowski,Jonathon A. Briggs,Jennifer C. Mortimer,Theodora Tryfona,Nicolas Terrapon,Elisabeth C. Lowe,Arnaud Baslé,Carl Morland,Alison M. Day,Hongjun Zheng,Theresa E. Rogers,Paul Thompson,Alastair R. Hawkins,Madhav P. Yadav,Bernard Henrissat,Eric C. Martens,Paul Dupree,Harry J. Gilbert,David N. Bolam +18 more
TL;DR: It is shown that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan.
Journal ArticleDOI
Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity
David N. Bolam,Antonio Ciruela,Simon J. McQueen-Mason,Peter J. Simpson,Michael P. Williamson,Jane E. Rixon,Alisdair B. Boraston,Geoffrey P. Hazlewood,Harry J. Gilbert +8 more
TL;DR: Pseudomonas CBDs did not induce the extension of isolated plant cell walls nor weaken cellulose paper strips in the same way as a class of plant cell wall proteins called expansins, suggesting that the CBD can accommodate at least six glucose molecules and has a much higher affinity for insoluble cellulose than soluble oligosaccharides.