Denis Fellmann

TL;DR: The primary automated data acquisition software system, Leginon, has been completely redesigned over the past two years and the system has demonstrated the capacity for high throughput data acquisition by acquiring images of more than 100,000 particles in a single session at the microscope.

TL;DR: The vision for this technique is to provide a straightforward manner in which users can proceed from raw data to a reliable 3D reconstruction through a pipeline that both facilitates management of the processing steps and makes the results at each step more transparent.

TL;DR: A particle selection "bakeoff" was included in the program of the Multidisciplinary Workshop on Automatic Particle Selection for cryoEM, and it was agreed that establishing benchmark particles and using bakeoffs to evaluate algorithms are useful in promoting algorithm development for fully automated particle selection.

TL;DR: The potential of the Leginon system for high-throughput data acquisition is demonstrated by describing an experiment in which more than 280,000 particles of GroEL are acquired in a single 25 h session at the microscope and the resolution of the reconstructed 3D density maps improves with decreasing ice thickness.

TL;DR: An improved holey carbon film has been developed that uses a hard template and a water-soluble release layer to replicate the template pattern into the carbon films and has a dramatic improvement on the distribution of single particles embedded in vitreous ice suspended across the holes.