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Scott M. Stagg
Researcher at Florida State University
Publications - 92
Citations - 5800
Scott M. Stagg is an academic researcher from Florida State University. The author has contributed to research in topics: COPII & COPI. The author has an hindex of 27, co-authored 80 publications receiving 5098 citations. Previous affiliations of Scott M. Stagg include Scripps Research Institute & University of Alabama at Birmingham.
Papers
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Journal ArticleDOI
Automated molecular microscopy: the new Leginon system.
Christian Suloway,James Pulokas,Denis Fellmann,Anchi Cheng,Francisco Guerra,Joel Quispe,Scott M. Stagg,Clinton S. Potter,Bridget Carragher +8 more
TL;DR: The primary automated data acquisition software system, Leginon, has been completely redesigned over the past two years and the system has demonstrated the capacity for high throughput data acquisition by acquiring images of more than 100,000 particles in a single session at the microscope.
Journal ArticleDOI
Appion: an integrated, database-driven pipeline to facilitate EM image processing.
Gabriel C. Lander,Scott M. Stagg,Neil R. Voss,Anchi Cheng,Denis Fellmann,James Pulokas,Craig Yoshioka,Christopher Irving,Anke M. Mulder,Pick-Wei Lau,Dmitry Lyumkis,Clinton S. Potter,Bridget Carragher +12 more
TL;DR: The vision for this technique is to provide a straightforward manner in which users can proceed from raw data to a reliable 3D reconstruction through a pipeline that both facilitates management of the processing steps and makes the results at each step more transparent.
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Incorporation of Aminoacyl-tRNA into the Ribosome as seen by Cryo-electron Microscopy
Mikel Valle,A. Zavialov,Wen Li,Scott M. Stagg,Jayati Sengupta,Rikke Nielsen,Poul Nissen,Stephen C. Harvey,Måns Ehrenberg,Joachim Frank,Joachim Frank +10 more
TL;DR: A cryo-electron microscopy study presents a mechanism that can explain the sequence of events during the decoding of mRNA on the ribosome, and proposes a mechanism to facilitate codon recognition by the incoming aa-tRNA and to provide the codon-anticodon recognition-dependent signal for the GTPase activity of EF-Tu.
Journal ArticleDOI
Structure of the Sec13/31 COPII coat cage
Scott M. Stagg,Cemal Gurkan,Douglas M. Fowler,Paul LaPointe,Ted R. Foss,Clinton S. Potter,Bridget Carragher,William E. Balch +7 more
TL;DR: This work shows that Sec13/31 can self-assemble to form minimal cages in the absence of Sec23/24, and reveals a novel cuboctahedron geometry with the potential to form a flexible lattice and to generate a diverse range of containers.
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Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.
Haixiao Gao,Jayati Sengupta,Mikel Valle,Andrei A. Korostelev,Narayanan Eswar,Scott M. Stagg,Patrick Van Roey,Rajendra K. Agrawal,Rajendra K. Agrawal,Stephen C. Harvey,Andrej Sali,Michael Chapman,Joachim Frank,Joachim Frank,Joachim Frank +14 more
TL;DR: Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.