Journal ArticleDOI
Automated molecular microscopy: the new Leginon system.
Christian Suloway,James Pulokas,Denis Fellmann,Anchi Cheng,Francisco Guerra,Joel Quispe,Scott M. Stagg,Clinton S. Potter,Bridget Carragher +8 more
TLDR
The primary automated data acquisition software system, Leginon, has been completely redesigned over the past two years and the system has demonstrated the capacity for high throughput data acquisition by acquiring images of more than 100,000 particles in a single session at the microscope.About:
This article is published in Journal of Structural Biology.The article was published on 2005-07-01. It has received 1505 citations till now. The article focuses on the topics: Data acquisition & Software system.read more
Citations
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Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein.
Alexandra C. Walls,Young-Jun Park,M. Alejandra Tortorici,M. Alejandra Tortorici,Abigail Wall,Andrew T. McGuire,Andrew T. McGuire,David Veesler +7 more
TL;DR: It is demonstrating that cross-neutralizing antibodies targeting conserved S epitopes can be elicited upon vaccination, and it is shown that SARS-CoV-2 S uses ACE2 to enter cells and that the receptor-binding domains of Sars- coV- 2 S and SARS S bind with similar affinities to human ACE2, correlating with the efficient spread of SATS among humans.
Journal ArticleDOI
RELION: implementation of a Bayesian approach to cryo-EM structure determination.
TL;DR: Developments that reduce the computational costs of the underlying maximum a posteriori (MAP) algorithm, as well as statistical considerations that yield new insights into the accuracy with which the relative orientations of individual particles may be determined are described.
Journal ArticleDOI
Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody.
Dora Pinto,Young-Jun Park,Martina Beltramello,Alexandra C. Walls,M. Alejandra Tortorici,M. Alejandra Tortorici,Siro Bianchi,Stefano Jaconi,Katja Culap,Fabrizia Zatta,Anna De Marco,Alessia Peter,Barbara Guarino,Roberto Spreafico,Elisabetta Cameroni,James Brett Case,Rita E. Chen,Colin Havenar-Daughton,Gyorgy Snell,Amalio Telenti,Herbert W. Virgin,Antonio Lanzavecchia,Michael S. Diamond,Katja Fink,David Veesler,Davide Corti +25 more
TL;DR: Several monoclonal antibodies that target the S glycoprotein of SARS-CoV-2, which was identified from memory B cells of an individual who was infected with severe acute respiratory syndrome coronavirus (SARS- coV) in 2003, and one antibody (named S309) potently neutralization, which may limit the emergence of neutralization-escape mutants.
Journal ArticleDOI
Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike.
Lihong Liu,Pengfei Wang,Manoj S. Nair,Jian Yu,Micah Rapp,Qian Wang,Yang Luo,Jasper Fuk-Woo Chan,Jasper Fuk-Woo Chan,Vincent Sahi,Amir Figueroa,Xinzheng V. Guo,Gabriele Cerutti,Jude Bimela,Jason Gorman,Tongqing Zhou,Zhiwei Chen,Zhiwei Chen,Kwok-Yung Yuen,Kwok-Yung Yuen,Peter D. Kwong,Peter D. Kwong,Joseph Sodroski,Michael T. Yin,Zizhang Sheng,Zizhang Sheng,Yaoxing Huang,Lawrence Shapiro,Lawrence Shapiro,David D. Ho +29 more
TL;DR: A diverse collection of potent neutralizing antibodies against the SARS-CoV-2 spike protein have been isolated from five patients with severe COVID-19 and high serum neutralization titres, suggesting both of these regions at the top of the viral spike are immunogenic.
Journal ArticleDOI
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.
Luca Piccoli,Young-Jun Park,M. Alejandra Tortorici,M. Alejandra Tortorici,Nadine Czudnochowski,Alexandra C. Walls,Martina Beltramello,Chiara Silacci-Fregni,Dora Pinto,Laura E. Rosen,John E. Bowen,Oliver J. Acton,Stefano Jaconi,Barbara Guarino,Andrea Minola,Fabrizia Zatta,Nicole Sprugasci,Jessica Bassi,Alessia Peter,Anna De Marco,Jay C. Nix,Federico Mele,Sandra Jovic,Blanca Fernandez Rodriguez,Sneha V. Gupta,Feng Jin,Giovanni Piumatti,Giovanni Piumatti,Giorgia Lo Presti,Alessandra Franzetti Pellanda,Maira Biggiogero,Maciej Tarkowski,Matteo Samuele Pizzuto,Elisabetta Cameroni,Colin Havenar-Daughton,Megan Smithey,David K. Hong,Valentino Lepori,Emiliano Albanese,Alessandro Ceschi,Alessandro Ceschi,Enos Bernasconi,Luigia Elzi,Paolo Ferrari,Christian Garzoni,Agostino Riva,G. Snell,Federica Sallusto,Katja Fink,Herbert W. Virgin,Antonio Lanzavecchia,Davide Corti,David Veesler +52 more
TL;DR: It is found that both the magnitude of Ab responses to SARS-CoV-2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores, and the immunodominance of the receptor-binding motif will guide the design of COVID-19 vaccines and therapeutics.
References
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Book
Tcl and the Tk Toolkit
TL;DR: The Addison-Wesley Publishing Company, Inc. as mentioned in this paper has published the first edition of this book, which is available for personal use only and requires prior written permission of the author or publisher.
Journal ArticleDOI
The crystal structure of the bacterial chaperonin GroEL at 2.8 A.
Kerstin Braig,Zbyszek Otwinowski,Zbyszek Otwinowski,Rashmi Hegde,Rashmi Hegde,David C Boisvert,Andrzej Joachimiak,Andrzej Joachimiak,Arthur L. Horwich,Paul B. Sigler +9 more
TL;DR: The crystal structure of Escherichia coli GroEL shows a porous cylinder of 14 subunits made of two nearly 7-fold rotationally symmetrical rings stacked back-to-back with dyad symmetry.
Journal ArticleDOI
Dual-axis tomography: an approach with alignment methods that preserve resolution.
TL;DR: Analysis by Fourier sector correlation indicated that the variable tilt increment improved the reconstruction in some respects but degraded it in others, and a varying tilt increment thus does not give an unqualified improvement, at least when using back-projection algorithms for the reconstruction.