Dhevahi Niranjan

TL;DR: Using live-cell confocal and single-molecule imaging of rat hippocampal neurons cultured in microfluidic devices, it is shown that the activity-dependent uptake of the binding domain of the BoNT/A heavy chain (BoNT/Hc) is followed by a delayed increase in retrograde axonal transport of BoNT-Hc carriers.

TL;DR: It is shown that SNARE tagging allows stepwise production of a functional modular medicinal toxin, namely, botulinum neurotoxin type A, commonly known as BOTOX, and that the SNARE tetrahelical coiled-coil allows controlled linking of various building blocks into multifunctional assemblies.

TL;DR: The stapled chimera inhibited mechanical hypersensitivity in a rat model of inflammatory pain without causing either flaccid or spastic paralysis and provides the first evidence that the protein stapling technology allows assembly of distinct proteins yielding new biomedical properties.

TL;DR: The results show that the re-assembled toxin represents a uniquely safe tool for neuroscience research and future medical applications and is potent in inhibiting CNS function at 1 nM concentration but surprisingly does not exhibit systemic toxicity after intraperitoneal injection even at 200 ng/kg dose.

TL;DR: At nanomolar concentrations, the retargeted botulinum molecules were able to inhibit stimulated release of hormones from tested cell lines suggesting their application for treatments of neuroendocrine disorders.