Showing papers in "Bioconjugate Chemistry in 2013"
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TL;DR: In vitro cytotoxicity experiments demonstrated that the h1F6239C-PBD was potent and immunologically specific on CD70-positive renal cell carcinoma (RCC) and non-Hodgkin lymphoma (NHL) cell lines, demonstrating that PBDs can be effectively used for antibody-targeted therapy.
232 citations
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TL;DR: This review article summarizes the enzymatic strategies, which enable site-specific functionalization of proteins with a variety of different functional groups, which include formylglycine generating enzyme, sialyl transferases, phosphopantetheinyltransferases, O-GlcNAc post-translational modification, sortagging, transglutaminase, farnesyltransferase, biotin ligase, lipoic acid ligase and N-myristoyltransferase
225 citations
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TL;DR: This review summarizes recent research results concerning the nature and density of head functional groups for improving the design of efficient cationic lipids to overcome the critical barriers of in vitro and in vivo transfection.
219 citations
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TL;DR: The reactivity of the fastest bioorthogonal reaction, the inverse-electron-demand-Diels-Alder cycloaddition between a tetrazine probe and a trans-cyclooctene-tagged antibody, can be increased 10-fold, approaching the speed of biological interactions, while also increasing its stability.
213 citations
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TL;DR: In this review, the state of the art of the different protein immobilization strategies that are commonly used today with a special focus on biosensor applications are discussed.
171 citations
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TL;DR: The proposed design of nanogel-drug conjugates allowed us to significantly enhance drug bioavailability, cancer cell targeting, and the treatment efficacy against drug-resistant cancer cells and multicellular spheroids.
164 citations
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TL;DR: A simple multifunctional nanoparticle system that can deliver DOX to the targeted cancer cells and enable us to localize the graphene-HQDs and monitor intracellular DOX release has been demonstrated in vitro.
164 citations
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TL;DR: The new hydrazino-Pictet-Spengler ligation, which has two distinct advantages over hydrazone and oxime ligations, exhibits a combination of product stability and speed near neutral pH that is unparalleled by current carbonyl bioconjugation chemistries.
139 citations
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TL;DR: The broad utility of this reaction in the chemoselective modification of small molecules, peptides, and proteins under mild aqueous conditions over a broad pH range using a wide variety of biologically acceptable buffers as well as others and mixed buffered compositions are supported.
132 citations
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TL;DR: The site-selective labeling methodology has proven facile, reproducible, and robust, producing (89)Zr-labeled radioimmunoconjguates that display high stability and immunoreactivity in vitro (>95%) in addition to highly selective tumor uptake in athymic nude mice bearing PSMA-expressing subcutaneous LNCaP xenografts.
129 citations
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TL;DR: Cumulatively, the results demonstrate that the proper tuning of QD dose, surface ligand, and delivery modality can provide robust in vitro cell labeling reagents that exhibit minimal impact on cellular viability.
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TL;DR: It is suggested that B6-NP might serve as a promising DDS for facilitating the brain delivery of neuropeptides in the treatment of Alzheimer's disease.
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TL;DR: Improved understanding from results was able to design a strategy to enhance the stability of DNA-AuNPs by conjugating double-stranded DNA to AuNPs through multiple thiol anchors.
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TL;DR: The discovery of a new catalyst that is up to 15 times more efficient than aniline is reported, which should have a significant impact on the field of bioconjugation, where oxime ligation and hydrazone-oxime exchange are commonly employed.
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TL;DR: This work teaches how to build effective hydroxamate-based sideromycins as Gram-positive selective antibiotic agents as well as novel antibiotic delivery technology with untapped potential for developing sophisticated microbe-selective antibacterial agents that limit the emergence of bacterial resistance.
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TL;DR: The synthesis of a complex nanoparticle (NP) system with dual drug loading as well as diagnostic properties is reported, which was found to accumulate at the tumor site by fluorescence imaging in tumor bearing mice intravenously administered NPs.
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TL;DR: A library of fucose analogues was evaluated and a thiolated analogue, 6-thiofucose, which was incorporated into the antibody carbohydrate with good efficiency and could be used for conjugation using maleimide chemistry to produce antibody-drug conjugates with pronounced cytotoxic activities and improved homogeneity compared to drug attachment through hinge disulfides.
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TL;DR: Combined photothermal therapy and chemotherapy with the paclitaxel-gold nanorod enabled photothermal chemotherapy has the potential of preventing tumor reoccurrence and metastasis and may have an important impact on the treatment of head and neck cancer and other malignancies in the clinic.
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TL;DR: This study explores the use of two tumor-specific targeting ligands to deliver near-infrared (NIR) fluorescent dyes specifically to FR and PSMA expressing cancers, thereby rendering only the malignant cells highly fluorescent.
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TL;DR: The effect of thermal stress on the physical stability, specifically aggregation, of an antibody drug conjugate (ADC), ADC 1, wherein the antibody was linked to the val-cit-Monomethyl Auristatin E (vc-MMAE) linker drug through the reduction of interchain disulfides was explored.
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TL;DR: It is reported that attachment of an anticancer drug doxorubicin to the N- or C-terminal of the Tat peptide can have a significant impact on their cellular uptake and cytotoxicity against both drug-sensitive and drug-resistant cancer cells.
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TL;DR: Collagen-specific staining methods using simple CMPs conjugated to common fluorophores allow direct detection of collagens and collagen-like proteins in SDS-PAGE and in various mammalian tissue sections and allow facile identification of pathologic conditions in fibrotic liver tissues.
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TL;DR: Directional antibody conjugation to the gold nanorod surface is introduced and molecular specificity of the synthesized antibody targeted nanorods was demonstrated using hyperspectral, optical and photoacoustic imaging of cancer cell culture models.
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TL;DR: Gemcitabine conjugated micelles were able to enhance the drug payload, protect it from rapid plasma metabolism, and provide a sustained release and showed enhanced antitumor activity, and thus have the potential to provide a better therapeutic alternative for treating pancreatic cancer.
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TL;DR: The aim of this review is to provide a comprehensive overview of the molecular imaging tracers developed for optical and nuclear identification of bacteria and bacterial infections and envision that such tracers can be used to diagnose infections and aid their clinical management.
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TL;DR: It is shown that cyclen-based paramagnetic lanthanide tags can be attached to proteins in a site-specific manner by Cu(I)-catalyzed azide-alkyne cycloaddition to a genetically encoded p-azido-l-phenylalanine residue with a tether that proved sufficiently short and rigid for the observation of PCSs in several proteins.
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TL;DR: It is demonstrated that functionalization of bioinorganic LDH with flexible polycation brushes is an effective means to produce new LDH-based gene delivery systems.
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TL;DR: This work investigates the ability of ligands against Treg-specific receptors to drive selective internalization of PEG-modified single-walled carbon nanotubes (PEG-SWCNTs) into Treg residing in the tumor microenvironment and hopes it will pave the way to innovative immunotherapies against cancer.
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TL;DR: This study sought to identify the effects of chemical conjugation on the electrostatic properties of Fc-conjugates by isolated a single Fc charge variant prior to conjugating of a fluorescent probe to the side chains of lysine residues.
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TL;DR: The effects of peptide valency, density, and affinity on nanoparticle delivery and therapeutic efficacy are examined, using the α(v)β(6)-specific H2009.1 peptide as a model phage-selected peptide and liposomal doxorubicin as a models therapeutic nanoparticle.