Dzifa Amengor

TL;DR: High-yield production of a stabilized prefusion spike protein will accelerate the development of vaccines and serological diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

TL;DR: Over 100 structure-guided spike variants based upon a previously determined cryo-EM structure of the prefusion SARS-CoV-2 spike are designed and expressed, with the best variant, HexaPro, having six beneficial proline substitutions leading to ∼10-fold higher expression than its parental construct.

TL;DR: The authors identified residues 980-1006 in the flexible hinge region at the S2 apex as the 3A3 epitope, consistent with binding to natural and engineered spike variants, which may help guide design of pan-protective spike immunogens.

TL;DR: In this article , recombinant antibodies were found to protect multiple pertactin epitopes against Bordetella pertussis infection and significantly reduced lung colonization levels after sub-lethal challenge, with no antibody mediating antibodydependent complement-induced lysis.

TL;DR: In this paper , a conformational S2 domain epitope present only in the prefusion core of β-coronavirus was described, and a third class of S2 antibody was defined.