E
Ellen Gielen
Researcher at Transnational University Limburg
Publications - 7
Citations - 411
Ellen Gielen is an academic researcher from Transnational University Limburg. The author has contributed to research in topics: Fluorescence correlation spectroscopy & Diffusion (business). The author has an hindex of 7, co-authored 7 publications receiving 400 citations. Previous affiliations of Ellen Gielen include Katholieke Universiteit Leuven & The Catholic University of America.
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Journal ArticleDOI
Probing Diffusion Laws within Cellular Membranes by Z-Scan Fluorescence Correlation Spectroscopy
Jana Humpolíčková,Ellen Gielen,Ellen Gielen,Aleš Benda,Veronika Fagul'ová,Jo Vercammen,Martin vandeVen,Martin Hof,Marcel Ameloot,Yves Engelborghs +9 more
TL;DR: This work describes an alternative approach that yields essentially the same information, and can readily be applied on commercial FCS instruments by measuring the diffusion time and the particle number at various relative positions of the cell membrane with respect to the waist of the laser beam, by performing a Z-scan.
Journal ArticleDOI
Rafts in oligodendrocytes: Evidence and structure–function relationship
TL;DR: An overview of the evidence supporting the presence of rafts in oligodendrocytes, the myelin‐producing cells of the central nervous system, is provided and their functional significance is discussed.
Journal ArticleDOI
Measuring diffusion of lipid-like probes in artificial and natural membranes by raster image correlation spectroscopy (RICS): use of a commercial laser-scanning microscope with analog detection.
Ellen Gielen,Ellen Gielen,Nick Smisdom,Martin vandeVen,Ben De Clercq,Enrico Gratton,Michelle A. Digman,Jean-Michel Rigo,Johan Hofkens,Yves Engelborghs,Marcel Ameloot +10 more
TL;DR: It is shown that RICS on a commercial CLSM with analog detection allows for reliable results in the study of membrane diffusion by removal of unwanted correlations introduced by the analog detection system.
Journal ArticleDOI
Cytokine-induced cell death in human oligodendroglial cell lines. II: Alterations in gene expression induced by interferon-gamma and tumor necrosis factor-alpha.
Mieke Buntinx,Ellen Gielen,Paul Van Hummelen,Jef Raus,Marcel Ameloot,Paul Steels,Piet Stinissen +6 more
TL;DR: CDNA microarray analysis of the HOG cell line revealed that TNF‐α induces genes that regulate apoptosis, survival, inflammation, cell metabolism, and cell signaling, which suggests that oligodendroglial cells activate both death and survival pathways upon cytokine challenges.
Journal ArticleDOI
Diffusion of myelin oligodendrocyte glycoprotein in living OLN-93 cells investigated by raster-scanning image correlation spectroscopy (RICS).
Ellen Gielen,Ellen Gielen,Nick Smisdom,Ben De Clercq,Ben De Clercq,Martin vandeVen,Rik Gijsbers,Zeger Debyser,Jean-Michel Rigo,Johan Hofkens,Yves Engelborghs,Marcel Ameloot +11 more
TL;DR: The first investigations of the diffusion behavior of the myelin-specific protein MOG (myelin oligodendrocyte glycoprotein) in OLN-93 as studied by the recently developed RICS (raster-scanning image correlation spectroscopy) technique are reported on.