Eric Macia

TL;DR: Dynamin acts at two steps during clathrin coat formation; GTP hydrolysis is probably needed at both steps; Dynasore acts as a potent inhibitor of endocytic pathways known to depend on dynamin by rapidly blocking coated vesicle formation within seconds of dynasore addition.

TL;DR: It is proposed that PI4KIIalpha establishes the Golgi's unique lipid-defined organelle identity by generating PI(4)P-rich domains that specify the docking of the AP-1 coat machinery.

TL;DR: A detailed synthesis protocol for dynasore is presented, and a series of experiments used to analyze the inhibitory effects of dyn asore on dynamin in vitro and to study the effects of Dynasore on endocytosis in cells are described.

TL;DR: The crystal structure of the core of the AP-1 complex, which functions in the trans-Golgi network (TGN), is reported and it is shown that directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells and diminish PI-4-P- dependent, but not Arf1-dependent, liposome binding in vitro.

TL;DR: The results show that the dual roles of PI4P can promote specific GGA targeting and cargo recognition at the TGN and has a novel role in promoting their recognition of the ubiquitin (Ub) sorting signal.