Tom Kirchhausen

TL;DR: It is proposed that tubular endosomes extend intracellularly and polarize towards the interacting T cell, but only when antigen-laden dendritic cells encounter T cells of the appropriate specificity, and serves to facilitate the ensuing T-cell response.

TL;DR: AO-LLSM takes high-resolution live-cell imaging of subcellular processes from the confines of the coverslip to the more physiologically relevant 3D environment within whole transparent organisms and creates new opportunities to study the phenotypic diversity of intracellular dynamics, extracellular communication, and collective cell behavior across different cell types, organisms, and developmental stages.

TL;DR: On demand, rapid Ca2+-triggered homotypic and exocytic membrane-fusion events are required to repair a torn plasma membrane, and it is proposed that this emergency-based fusion differs fundamentally from other rapid, triggered fusion reactions.

TL;DR: This review describes the structures of clathrin, major cargo adaptors, and other proteins that participate in forming aClathrin-coated pit, loading its contents, pinching off the membrane as a lattice-enclosed vesicle, and recycling the components.

TL;DR: A detailed synthesis protocol for dynasore is presented, and a series of experiments used to analyze the inhibitory effects of dyn asore on dynamin in vitro and to study the effects of Dynasore on endocytosis in cells are described.