Steeve Veronneau

TL;DR: It is shown that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment, and less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal Growth in four of the tested conditions.

TL;DR: A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome), finding that 17 percent were essential for viability in rich medium.

TL;DR: The combination of phenotypic and bioinformatic analyses further improves drug target selection from the C. albicans essential gene set, and their respective conditional mutant strains may be directly used as sensitive whole‐cell assays for drug screening.

TL;DR: The role of the three genes most closely related to MNN1p in the addition of the fourth and fifth alpha-1,3-linked mannose residues to Man4 and Man5 oligosaccharides was examined in mutants carrying single and multiple combinations of the disrupted genes as mentioned in this paper.

TL;DR: It is concluded that depolarization of the actin cytoskeleton is a specific phenotype and that slow growth alone is not sufficient to causeDepolarization.