Fu Shujin

TL;DR: The observations suggest that the BGISEQ-500 holds the potential to represent a valid and potentially valuable alternative platform for palaeogenomic data generation that is worthy of future exploration by those interested in the sequencing and analysis of degraded DNA.

TL;DR: The results indicate the potential applicability of the BGISEQ-500 platform for the identification of somatic and germline single nucleotide variants by whole genome sequencing, and represent the first publicly-available cancer genome sequencing performed using this platform.

TL;DR: In this article, a sequencing library building method and reagent based on a molecular inverse probe was proposed, which includes using denatured nucleic acid to perform annealing hybridization, where the inverse probe comprises the anchoring sequence of a 5' end, the extension sequence of 3' end and the sequencing connector sequence between the anchor and the extension sequences, and the anchors and extensions are respectively inversely complementary with sequences at two ends of a target area.

TL;DR: In this article, a linker element and a method to construct a sequencing library is presented, where the linker elements are used to solve the problem of fragment interlinking, linker self-linking and low linking efficiency, and reduce the purification reaction between steps.

TL;DR: In this article, a library construction method for invasive antenatal testing and a kit is presented, which includes steps of performing tail end repairing and A adding on segmental DNA by using polymerase, polynucleotide kinase and polymerase without 3'-5' excision activity in the same reaction system in the presence of dNTP and additional dATP; then performing joint adding and purification, PCR and looping.