H
Helge Ewers
Researcher at Free University of Berlin
Publications - 87
Citations - 5053
Helge Ewers is an academic researcher from Free University of Berlin. The author has contributed to research in topics: Microscopy & Super-resolution microscopy. The author has an hindex of 29, co-authored 75 publications receiving 4255 citations. Previous affiliations of Helge Ewers include École Polytechnique Fédérale de Lausanne & King's College London.
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Posted ContentDOI
Glypican-1 drives unconventional secretion of Fibroblast Growth Factor 2
Carola Sparn,Eleni Dimou,Annalena Meyer,Roberto Saleppico,Sabine Wegehingel,Matthias Gerstner,Severina Klaus,Helge Ewers,Walter Nickel +8 more
TL;DR: In this paper, the structural basis for GPC1-dependent FGF2 secretion was identified, identifying disaccharides with N-linked sulfate groups to be enriched in the heparan sulfate chains of GPC 1 to which FGF 2 binds with high affinity.
Posted ContentDOI
Revealing compartmentalised membrane diffusion in living cells with interferometric scattering microscopy
TL;DR: Interferometric scattering microscopy (iSCAT) combined with gold nanoparticle labeling can be used to follow the motion of membrane proteins in the plasma membrane of live cultured mammalian cell lines and hippocampal neurons and reveals signatures of a compartmentalised plasma membrane in neurons.
Journal ArticleDOI
3D surface reconstruction of cellular cryo-soft X-ray microscopy tomograms using semisupervised deep learning
Michael Ca Dyhr,Mohsen Sadeghi,Ralitsa Moynova,Carolin Knappe,S. Werner,Gerd Schneider,Frank Noé,Helge Ewers +7 more
TL;DR: In this article , a convolutional neural network is used to segment the 3D ultrastructural detail of cells in their near-native state at high resolution for the segmentation of CryoSoft X-ray tomography (cryo-SXT) datasets.
Book ChapterDOI
Ashbya gossypii as a model system to study septin organization by single-molecule localization microscopy.
TL;DR: This protocol provides a protocol that enables the investigation of the organization of septin complexes in higher order structures in cells by combining advantageous features of the model organism Ashbya gossypii with single-molecule localization microscopy.
Posted ContentDOI
Expansion stimulated emission depletion microscopy (ExSTED)
Mengfei Gao,Riccardo Maraspini,Oliver Beutel,Amin Zehtabian,Britta J. Eickholt,Alf Honigmann,Helge Ewers +6 more
TL;DR: This work provides a robust template for super resolution microscopy of entire cells in the ten nanometer range and finds that high fidelity labelling via multi-epitopes is required to obtain emitter densities that allow to resolve ultra-structural details with ExSTED.