H
Helge Ewers
Researcher at Free University of Berlin
Publications - 87
Citations - 5053
Helge Ewers is an academic researcher from Free University of Berlin. The author has contributed to research in topics: Microscopy & Super-resolution microscopy. The author has an hindex of 29, co-authored 75 publications receiving 4255 citations. Previous affiliations of Helge Ewers include École Polytechnique Fédérale de Lausanne & King's College London.
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Journal ArticleDOI
Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy
Charlotte Kaplan,Bo Jing,Christian M. Winterflood,Andrew A. Bridges,Patricia Occhipinti,Jürgen J. Schmied,Sören Grinhagens,Thomas Gronemeyer,Philip Tinnefeld,Amy S. Gladfelter,Jonas Ries,Helge Ewers +11 more
TL;DR: The organization of subunits in cytoskeletal polymers in cells is resolved by light microscopy and cellular septins were resolved as localization pairs and thin stretches of equidistant localizations.
Journal ArticleDOI
Dual color single particle tracking via nanobodies
TL;DR: A simple dual color single particle tracking assay is demonstrated using small, bright, high-affinity labeling via nanobodies of accessible targets with widely available instrumentation to visualize differences in apparent membrane viscosity.
Posted ContentDOI
Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells
Christian H. Gabriel,Marta del Olmo,Amin Zehtabian,Silke Reischl,Hannah van Dijk,Barbara Koller,Astrid Grudziecki,Bert Maier,Helge Ewers,Hanspeter Herzel,Adrián E. Granada,Achim Kramer +11 more
TL;DR: This paper describes a new strategy for the efficient generation of knock-in reporter cell lines using CRISPR technology that is particularly useful for lowly or transiently expressed genes, such as those coding for circadian clock proteins.
Journal ArticleDOI
Inhibition of sphingolipid synthesis affects kinetics but not fidelity of L1/NgCAM transport along direct but not transcytotic axonal pathways.
TL;DR: The results yield a mechanism for the axon outgrowth defect observed in FB1, and it is shown that reducing raft lipid levels does not disrupt axonal targeting of L1/NgCAM along either pathway.
Journal ArticleDOI
Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex
TL;DR: The four-transmembrane-spanning protein tetraspanin-3 (TSPAN3) is identified as a new modulatory co-receptor for the Nogo-A inhibitory domain Nogo -A-Δ20, and is required during Nogo’s cell spreading and inhibition of neurite outgrowth.