Jesse D. Marshall

TL;DR: Replacement of CFP and YFP with these two proteins in reporters of kinase activity, small GTPase activity and transmembrane voltage significantly improves photostability, FRET dynamic range and emission ratio changes and enhances detection of transient biochemical events.

TL;DR: Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential, demonstrated on and off kinetics and reliably detected single action potentials and subthreshold potential changes.

TL;DR: Findings support a supervised learning model in which activation of the US representation guides the transformation of the CS representation, and the strength of the ensemble-encoded CS–US association predicted the level of behavioural conditioning in each mouse.

TL;DR: Both the spatiotemporal profiles and rates of SPN activity appear crucial to striatal function, and next-generation treatments for basal ganglia disorders should target both facets of striatal activity.

TL;DR: New fluorescent proteins with properties better suited to a wide range of FRET applications are developed, including a voltage sensor that allows more reliable detection of single action potentials than do previous sensors and an improved RhoA reporter able to detect local and rapid RHoA activation in neuronal growth cones during ephrinA-stimulated retraction.