Yiyang Gong

TL;DR: Replacement of CFP and YFP with these two proteins in reporters of kinase activity, small GTPase activity and transmembrane voltage significantly improves photostability, FRET dynamic range and emission ratio changes and enhances detection of transient biochemical events.

TL;DR: Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential, demonstrated on and off kinetics and reliably detected single action potentials and subthreshold potential changes.

TL;DR: In vivo imaging revealed sensory-evoked responses, including somatic spiking, dendritic dynamics, and intracellular voltage propagation, which empower in vivo optical studies of neuronal electrophysiology and coding and motivate further advancements in high-speed microscopy.

TL;DR: Sensors that use fluorescence resonance energy transfer (FRET) to combine the rapid kinetics and substantial voltage-dependence of rhodopsin family voltage-sensing domains with the brightness of genetically engineered protein fluorophores are described.

TL;DR: New fluorescent proteins with properties better suited to a wide range of FRET applications are developed, including a voltage sensor that allows more reliable detection of single action potentials than do previous sensors and an improved RhoA reporter able to detect local and rapid RHoA activation in neuronal growth cones during ephrinA-stimulated retraction.