K
Katharine H.D. Crawford
Researcher at Fred Hutchinson Cancer Research Center
Publications - 34
Citations - 6655
Katharine H.D. Crawford is an academic researcher from Fred Hutchinson Cancer Research Center. The author has contributed to research in topics: Epitope & Antibody. The author has an hindex of 18, co-authored 28 publications receiving 3162 citations. Previous affiliations of Katharine H.D. Crawford include University of Washington.
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Journal ArticleDOI
Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding.
Tyler N. Starr,Allison J. Greaney,Allison J. Greaney,Sarah K Hilton,Sarah K Hilton,Daniel Ellis,Katharine H.D. Crawford,Katharine H.D. Crawford,Adam S. Dingens,Mary Jane Navarro,John E. Bowen,M. Alejandra Tortorici,Alexandra C. Walls,Neil P. King,David Veesler,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom +17 more
TL;DR: It is found that a substantial number of mutations to the RBD are well tolerated or even enhance ACE2 binding, including at ACE2 interface residues that vary across SARS-related coronaviruses.
Journal ArticleDOI
Comprehensive mapping of mutations in the SARS-CoV-2 receptor-binding domain that affect recognition by polyclonal human plasma antibodies.
Allison J. Greaney,Allison J. Greaney,Andrea N. Loes,Andrea N. Loes,Katharine H.D. Crawford,Katharine H.D. Crawford,Tyler N. Starr,Tyler N. Starr,Keara D. Malone,Helen Y. Chu,Jesse D. Bloom,Jesse D. Bloom +11 more
TL;DR: In this article, the authors map how convalescent plasma antibodies are impacted by all mutations to the spike's receptor-binding domain (RBD), the main target of plasma neutralizing activity.
Journal ArticleDOI
Complete Mapping of Mutations to the SARS-CoV-2 Spike Receptor-Binding Domain that Escape Antibody Recognition.
Allison J. Greaney,Allison J. Greaney,Tyler N. Starr,Pavlo Gilchuk,Seth J. Zost,Elad Binshtein,Andrea N. Loes,Andrea N. Loes,Sarah K Hilton,John Huddleston,Rachel Eguia,Katharine H.D. Crawford,Katharine H.D. Crawford,Adam S. Dingens,Rachel S. Nargi,Rachel E. Sutton,Naveenchandra Suryadevara,Paul W. Rothlauf,Paul W. Rothlauf,Zhuoming Liu,Sean P. J. Whelan,Robert H. Carnahan,Robert H. Carnahan,James E. Crowe,James E. Crowe,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom +27 more
TL;DR: A deep mutational scanning method is described to map how all amino-acid mutations in the RBD affect antibody binding, and this method is applied to 10 human monoclonal antibodies to enable rational design of antibody therapeutics and assessment of the antigenic consequences of viral evolution.
Journal ArticleDOI
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.
Katharine H.D. Crawford,Katharine H.D. Crawford,Rachel Eguia,Adam S. Dingens,Andrea N. Loes,Keara D. Malone,Caitlin R Wolf,Helen Y. Chu,M. Alejandra Tortorici,M. Alejandra Tortorici,David Veesler,Michael L. M. Murphy,Deleah Pettie,Neil P. King,Alejandro B. Balazs,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom +17 more
TL;DR: It is demonstrated how these pseudotyped lentiviral particles could be used to measure the neutralizing activity of human sera or plasma against SARS-CoV-2 in convenient luciferase-based assays, thereby providing a valuable complement to ELISA-based methods that measure antibody binding rather than neutralization.
Journal ArticleDOI
Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans during a Fishery Vessel Outbreak with a High Attack Rate.
Amin Addetia,Katharine H.D. Crawford,Katharine H.D. Crawford,Adam S. Dingens,Haiying Zhu,Pavitra Roychoudhury,Pavitra Roychoudhury,Meei Li Huang,Meei Li Huang,Keith R. Jerome,Keith R. Jerome,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom,Alexander L. Greninger,Alexander L. Greninger +15 more
TL;DR: The development of vaccines against SARS-CoV-2 would be greatly facilitated by the identification of immunological correlates of protection in humans, as the presence of neutralizing antibodies from prior infection was significantly associated with protection against reinfection.