K
Kathryn J. Hofmann
Researcher at Merck & Co.
Publications - 40
Citations - 971
Kathryn J. Hofmann is an academic researcher from Merck & Co.. The author has contributed to research in topics: Gene & Saccharomyces cerevisiae. The author has an hindex of 16, co-authored 40 publications receiving 966 citations.
Papers
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Journal ArticleDOI
Expression and secretion in yeast of a 400-kDa envelope glycoprotein derived from Epstein-Barr virus.
Schultz Loren D,Jerry Tanner,Kathryn J. Hofmann,Emilio A. Emini,Jon H. Condra,Raymond E. Jones,Elliott Kieff,Ronald W. Ellis +7 more
TL;DR: The major envelope glycoprotein (gp350) of Epstein-Barr virus has been expressed and secreted in the yeast Saccharomyces cerevisiae as a 400-kDa glycop protein, the first example of the secretion of such a large, heavily glycosylated heterologous protein in yeast.
Journal ArticleDOI
Human Papillomavirus Type 11 (HPV-11) Neutralizing Antibodies in the Serum and Genital Mucosal Secretions of African Green Monkeys Immunized with HPV-11 Virus-like Particles Expressed in Yeast
Robert S. Lowe,Darron R. Brown,Janine T. Bryan,Cook James C,George Hugh A,Kathryn J. Hofmann,William M. Hurni,Joseph G. Joyce,E. Dale Lehman,Henry Z. Markus,Neeper Michael P,Schultz Loren D,Alan R. Shaw,Kathrin U. Jansen +13 more
TL;DR: Protection against HPV infection of the uterine cervix may be possible through systemic immunization with HPV VLPs.
Journal ArticleDOI
Unusually high-level expression of a foreign gene (hepatitis B virus core antigen) in Saccharomyces cerevisiae.
Peter J. Kniskern,Arpi Hagopian,Donna L. Montgomery,Pamela D Burke,N. R. Dunn,Kathryn J. Hofmann,William J. Miller,Ronald W. Ellis +7 more
TL;DR: Analysis of the HBcAg coding sequence reveals a very low index of codon bias for S. cerevisiae, largely discounting codon usage as a contributor to the high level of protein obtained, which is explicable in part on the basis of the very high stability in yeast cells ofHBcAg polypeptides.
Journal ArticleDOI
Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast.
Schultz Loren D,Kathryn J. Hofmann,Lawrence M. Mylin,Donna L. Montgomery,Ronald W. Ellis,James E. Hopper +5 more
TL;DR: A novel yeast strain is constructed which overproduces the GAL4 protein in a regulated fashion and should prove generally useful for the maximal, regulated expression in yeast of structural genes driven by a galactose-inducible promoter.
Patent
EXPRESSION OF HUMAN INTERLEUKIN-1-g(b) IN A TRANSGENIC ANIMAL
Howard Y. Chen,Kathryn J. Hofmann,Der Ploeg Leonardus H. T. Van,Alan R. Shaw,Myrna E. Trumbauer,Hui Zheng +5 more
TL;DR: Transgenic non-human animals with a human interleukin 1β gene under control of the murine metallothionein-1 promoter were provided in this article. But the authors did not consider the effects of the gene expression on the human brain.