Keitaro Umezawa

TL;DR: A biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques, and its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues.

TL;DR: Reversible fluorescent probes for intracellular glutathione (GSH) imaging have now been designed and synthesized based on Si-rhodamine fluorophores and are shown to be capable of quantifying the GSH concentration in various living cell types and also for monitoring real-time live-cell imaging of GSH dynamics with a temporal resolution of seconds.

TL;DR: In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding the capacity for imaging living cells.

TL;DR: This review summarizes progress in the field of near-infrared (NIR) fluorophores achieved during the last decade from the viewpoint of chemistry, with particular focus on the recent developments of BODIPY and rhodamine derivatives.

TL;DR: Novel functional interactions among a set of 31 centrosomal proteins are revealed and it is identified that Cep57, Cep63, and Cep152 are parts of a ring-like complex localizing around the proximal end of centrioles, where the cartwheel is located.