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Kevin P. O’Rourke
Researcher at Memorial Sloan Kettering Cancer Center
Publications - 20
Citations - 2675
Kevin P. O’Rourke is an academic researcher from Memorial Sloan Kettering Cancer Center. The author has contributed to research in topics: Colorectal cancer & Metastasis. The author has an hindex of 11, co-authored 18 publications receiving 1999 citations. Previous affiliations of Kevin P. O’Rourke include Middlebury College & Rockefeller University.
Papers
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Journal ArticleDOI
Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration
Caroline A. Lindemans,Caroline A. Lindemans,Marco Calafiore,Anna Mertelsmann,Margaret O'Connor,Jarrod A Dudakov,Jarrod A Dudakov,Robert R. Jenq,Enrico Velardi,Lauren F. Young,Odette M. Smith,Gillian Lawrence,Juliet Ivanov,Ya-Yuan Fu,Shuichiro Takashima,Guoqiang Hua,Maria Laura Martin,Kevin P. O’Rourke,Yuan-Hung Lo,Michal Mokry,Monica Romera-Hernandez,Tom Cupedo,Lukas E. Dow,Edward E. S. Nieuwenhuis,Noah F. Shroyer,Chen Liu,Richard Kolesnick,Marcel R.M. van den Brink,Alan M. Hanash +28 more
TL;DR: It is shown that innate lymphoid cells (ILCs), potent producers of interleukin-22 (IL-22) after intestinal injury, increase the growth of mouse small intestine organoids in an IL-22-dependent fashion, revealing a fundamental mechanism by which the immune system is able to support the intestinal epithelium, activating ISCs to promote regeneration.
Journal ArticleDOI
Inducible in vivo genome editing with CRISPR-Cas9
Lukas E. Dow,Lukas E. Dow,Jonathan Fisher,Kevin P. O’Rourke,Kevin P. O’Rourke,Ashlesha Muley,Edward R. Kastenhuber,Edward R. Kastenhuber,Geulah Livshits,Darjus F. Tschaharganeh,Nicholas D. Socci,Scott W. Lowe +11 more
TL;DR: It is shown that doxycycline-regulated Cas9 induction enables widespread gene disruption in multiple tissues and that limiting the duration of Cas9 expression or using a Cas9D10A (Cas9n) variant can regulate the frequency and size of target gene modifications, respectively.
Journal ArticleDOI
Apc Restoration Promotes Cellular Differentiation and Reestablishes Crypt Homeostasis in Colorectal Cancer
Lukas E. Dow,Kevin P. O’Rourke,Kevin P. O’Rourke,Janelle Simon,Darjus F. Tschaharganeh,Johan H. van Es,Hans Clevers,Scott W. Lowe +7 more
TL;DR: It is revealed that CRC cells can revert to functioning normal cells given appropriate signals and provide compelling in vivo validation of the Wnt pathway as a therapeutic target for treatment of CRC.
Journal ArticleDOI
Cytokinesis failure triggers hippo tumor suppressor pathway activation.
Neil J. Ganem,Hauke Cornils,Shang-Yi Chiu,Kevin P. O’Rourke,Jonathan Arnaud,Dean Yimlamai,Manuel Théry,Fernando D. Camargo,David Pellman +8 more
TL;DR: It is demonstrated that cytokinesis failure activates the Hippo tumor suppressor pathway in cultured cells, as well as in naturally occurring tetraploid cells in vivo, defining an important tumor suppression mechanism and uncover adaptive mechanisms potentially available to nascent tumor cells that bypass this inhibitory regulation.
Journal ArticleDOI
A rectal cancer organoid platform to study individual responses to chemoradiation.
Karuna Ganesh,Chao Wu,Kevin P. O’Rourke,Bryan C. Szeglin,Youyun Zheng,Charles Etienne Gabriel Sauvé,Mohammad Adileh,Isaac Wasserman,Michael R. Marco,Amanda S. Kim,Maha Shady,Francisco Sanchez-Vega,Wouter R. Karthaus,Helen Won,Seo Hyun Choi,Raphael Pelossof,Afsar Barlas,Peter Ntiamoah,Emmanouil P. Pappou,Arthur E. Elghouayel,James S. Strong,Chin Tung Chen,Jennifer W. Harris,Martin R. Weiser,Garrett M. Nash,Jose G. Guillem,Iris H Wei,Richard Kolesnick,Harini Veeraraghavan,Eduardo J. Ortiz,Iva Petkovska,Andrea Cercek,Katia Manova-Todorova,Leonard B. Saltz,Jessica A. Lavery,Ronald P. DeMatteo,Joan Massagué,Philip B. Paty,Rona Yaeger,Xi Chen,Sujata Patil,Hans Clevers,Michael F. Berger,Scott W. Lowe,Jinru Shia,Paul B. Romesser,Lukas E. Dow,Julio Garcia-Aguilar,Charles L. Sawyers,J. Joshua Smith +49 more
TL;DR: The biology and drug sensitivity of RC clinical isolates can be efficiently interrogated using an organoid-based, ex vivo platform coupled with in vivo endoluminal propagation in animals.