L
Leslie Johnston-Dow
Researcher at Applied Biosystems
Publications - 12
Citations - 503
Leslie Johnston-Dow is an academic researcher from Applied Biosystems. The author has contributed to research in topics: Typing & Antigen. The author has an hindex of 10, co-authored 12 publications receiving 499 citations. Previous affiliations of Leslie Johnston-Dow include PerkinElmer.
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Journal ArticleDOI
Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3
A. Martin-Gallardo,W. R. McCombie,Jeannine D. Gocayne,Michael G. FitzGerald,S. Wallace,Brian M. Lee,Jane Lamerdin,S Trapp,Jenny M. Kelley,Luyang Liu,Mark Dubnick,Leslie Johnston-Dow,Anthony R. Kerlavage,P. De Jong,Anthony V. Carrano,Chris Fields,J. C. Venter +16 more
TL;DR: A total of 116, 118 basepairs derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods.
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Structural heterogeneity in HLA‐B70, a high‐frequency antigen of black populations *
John D. Domena,Ann-Margaret Little,A J Madrigal,William H. Hildebrand,Leslie Johnston-Dow,E. D. du Toit,Wilma B. Bias,Peter Parham +7 more
TL;DR: Cloning and sequencing of cDNA encoding B70 antigens from six cell lines has identified a group of three closely related alleles that form a subgroup of the B15 family that are close to that of the HLA-B consensus consistent with the difficulty in their serological definition.
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Heterozygote and Mutation Detection by Direct Automated Fluorescent DNA Sequencing Using a Mutant Taq DNA Polymerase
TL;DR: The method produces sequence ladders from unpurified PCR fragments of sufficiently high quality such that heterozygotes can be reproducibly detected and identified by software that recognizes signal-strength patterns indicative of mixed-base positions.
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A generic sequencing based typing approach for the identification of HLA-A diversity.
Sitha A. Scheltinga,Leslie Johnston-Dow,Camille B. White,Anne-Wil van der Zwan,Jantine E. Bakema,Erik H. Rozemuller,Jan G. van den Tweel,Mel N. Kronick,Marcel G.J. Tilanus +8 more
TL;DR: The SBT approach described here uses a locus specific amplification of DNA from exon 1 to exon 5 to identify new alleles directly and an identical new "HLA-A*0103" was identified in two Caucasian samples.
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HLA-B alleles associated with the B15 serologically defined antigens.
Noriko Steiner,Jennifer Ng,Janet Bush,Robert J. Hartzman,Leslie Johnston-Dow,Carolyn Katovich Hurley +5 more
TL;DR: The B15 unsplit and B75 groups were the most complex exhibiting 16 and 7 alleles, respectively, within each serotype, illustrating the complexity of this family.