The talk with present the key results of the IPCC Working Group III 5th assessment report. Concluding four years of intense scientific collaboration by hundreds of authors from around the world, the report responds to the request of the world's governments for a comprehensive, objective and policy neutral assessment of the current scientific knowledge on mitigating climate change. The report has been extensively reviewed by experts and governments to ensure quality and comprehensiveness.

Climate change 2014 - Mitigation of climate change

Climate change 2014 - Synthesis Report

Terrestrial net primary production (NPP) quantifies the amount of atmospheric carbon fixed by plants and accumulated as biomass. Previous studies have shown that climate constraints were relaxing with increasing temperature and solar radiation, allowing an upward trend in NPP from 1982 through 1999. The past decade (2000 to 2009) has been the warmest since instrumental measurements began, which could imply continued increases in NPP; however, our estimates suggest a reduction in the global NPP of 0.55 petagrams of carbon. Large-scale droughts have reduced regional NPP, and a drying trend in the Southern Hemisphere has decreased NPP in that area, counteracting the increased NPP over the Northern Hemisphere. A continued decline in NPP would not only weaken the terrestrial carbon sink, but it would also intensify future competition between food demand and proposed biofuel production.

Drought-Induced Reduction in Global Terrestrial Net Primary Production from 2000 Through 2009

INTRODUCTION: The copy number and in- tracellular localization of RNA are important regulators of gene expression. Measurement of these properties at the transcriptome scale in single cells will give answers to many ques- tions related to gene expression and regulation. Single-molecule RNA imaging approaches, such as single-molecule fluorescence in situ hybrid- ization(smFISH), are powerful toolsforcount- ing and mappingRNA; however, the number of RNA species that can be simultaneously im- aged in individual cells has been limited. This makes it challenging to perform transcriptomic analysis of single cells in a spatially resolved manner. Here, we report multiplexed error- robust FISH (MERFISH), a single-molecule im- aging method that allows thousands of RNA species to be imaged in single cells by using combinatorial FISH labeling with encoding schemes capable of detecting and/or correct- ing errors. RATIONALE: We labeled each cellular RNA with a set of encoding probes, which contain targeting sequences that bind the RNA and readout sequences that bind fluorescently la- beled readout probes. Each RNA species is encodedwithaparticular combinationofread- out sequences. We used successive rounds of hybridization and imaging, each with a differ- ent readout probe, to identify the readout se- quences bound to each RNA and to decode the RNA. In principle, combinatorial labeling al- lows the number of detectable RNA species to

http://science.sciencemag.org/content/sci/348/6233/aaa6090.full.pdf

Spatially resolved, highly multiplexed RNA profiling in single cells

Statistics and Data Analysis in Geology

Agriculture is a significant contributor to phosphorus (P) enrichment in aquatic ecosystems. No-till (NT) farming has been proposed as an alternative approach to conventional tillage (CT) in reducing soil P export, but published data have shown contrasting impacts, likely due to the interacting effects of different physical (climate region, rainfall variability, transport pathway, slope gradient) and management variables (NT duration, crop species). We conducted a meta-analysis to understand the extent to which each of these variables controls the concentration and load of different P fractions (dissolved P, particulate P) in agricultural runoff and leaching. In comparison with CT, particulate P loss was significantly lower with NT adoption (45 and 55% reduction in concentration and load, respectively), but an increase in dissolved P loss was observed. The extent of the reduction or increase, however, varied with different physical and management variables. In comparison with CT, for example, NT was not effective in reducing particulate P concentration during wet years and particulate P load on steep slopes (4-9%). Total P concentration was also similar with CT at sites under prolonged NT duration (∼10 yr) and at NT fields planted with soybean [ (L.) Merr.]. Our results underscore the need to consider the covarying physical and management factors when assessing the potential of NT farming in controlling P loss in the environment. The limited impact of NT on dissolved P loss remains a serious impediment toward harnessing the water quality benefits of this management practice.

Meta-Analysis of Phosphorus Loss from No-Till Soils

/pdf/forests-affected-by-frequent-and-intense-typhoons-challenge-wd24k3xvi4.pdf

Forests affected by frequent and intense typhoons challenge the intermediate disturbance hypothesis

https://esajournals.onlinelibrary.wiley.com/doi/pdf/10.1002/ecs2.1938

Tropical cyclones disrupt the relationship between tree height and species diversity: Comment

Cancer evolves from premalignant clones that accumulate mutations and adopt unusual cell states to achieve transformation. Previously, we pinpointed the oligodendrocyte precursor cell (OPC) as a cell-of-origin for glioma, but the early changes of mutant OPCs during premalignancy remained unknown. Using mice engineered for inducible Nf1-Trp53 loss in
OPCs, we acutely isolated labeled mutant OPCs by laser-capture microdissection and determined gene-expression changes by bulk RNA sequencing and a fluctuation analysis, called stochastic profiling, which uses RNA-sequencing measurements from random pools of 10 mutant cells. At 12 days after Nf1-Trp53 deletion, while bulk differences were mostly limited to
mitotic hallmarks and genes for ribosome biosynthesis, stochastic profiling revealed a spectrum of stem-progenitor (Axl, Aldh1a1), proneural, and mesenchymal states as potential starting points for gliomagenesis. At 90 days, bulk sequencing detected very few differentially expressed transcripts, whereas stochastic profiling revealed cell states for neurons and mural
cells that do not give rise to glial tumors, suggesting cellular dead-ends for gliomagenesis. Importantly, we identified mutant OPCs that strongly expressed key effectors of nonsense-mediated decay (Upf3b) and homology-dependent DNA repair (Rad51c, Slx1b, Ercc4) along
with DNA-damage markers suggesting transcription-associated replication stress. Analysis of 10-cell transcriptomes at 90 days identified a locus of elevated gene expression containing an additional repair endonuclease (Mus81) and Rin1, a Ras-Raf antagonist and possible counterbalance to Nf1 loss. At 150 days, Rin1 was microdeleted in some gliomas and downregulated in all others. Replication stress may pose a considerable bottleneck that must
be resolved for gliomas to initiate.

https://www.biorxiv.org/content/biorxiv/early/2021/01/19/2020.03.30.017228.full.pdf

Premalignant Nf1,Trp53-null Oligodendrocyte Precursor Cells Become Stalled in a Heterogeneous State of Replication Stress Before Gliomagenesis

Chinese fir, Cunninghamia lanceolata (Lamb.) Hook. (Taxodiaceae), is an evergreen conifer primarily distributed in southern China. This species exhibits very poor natural regeneration, possibly due...

/pdf/seedling-emergence-and-early-growth-of-chinese-fir-under-2lvpup2nzu.pdf

Lixin Wang

Papers

Meta-Analysis of Phosphorus Loss from No-Till Soils

Forests affected by frequent and intense typhoons challenge the intermediate disturbance hypothesis

Tropical cyclones disrupt the relationship between tree height and species diversity: Comment

Premalignant Nf1,Trp53-null Oligodendrocyte Precursor Cells Become Stalled in a Heterogeneous State of Replication Stress Before Gliomagenesis

Seedling emergence and early growth of Chinese fir under different light levels and seed positions: implications for natural regeneration