M
Masatoshi Hagiwara
Researcher at Kyoto University
Publications - 286
Citations - 17513
Masatoshi Hagiwara is an academic researcher from Kyoto University. The author has contributed to research in topics: Protein kinase A & RNA splicing. The author has an hindex of 60, co-authored 272 publications receiving 16297 citations. Previous affiliations of Masatoshi Hagiwara include University of Tokyo & Tokyo Medical and Dental University.
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Journal ArticleDOI
Phosphorylated CREB binds specifically to the nuclear protein CBP
John C. Chrivia,Roland P. S. Kwok,Ned J.C. Lamb,Masatoshi Hagiwara,Marc Montminy,Richard H. Goodman +5 more
TL;DR: It is proposed that CBP may participate in cAMP-regulated gene expression by interacting with the activated phosphorylated form of CREB, which is activated as a result of phosphorylation by protein kinase A7.
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Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells.
Takashi Chijiwa,Atsushi Mishima,Masatoshi Hagiwara,Mamoru Sano,Kyozo Hayashi,Tsutomu Inoue,Naito Kenji,Tadashi Toshioka,Hiroyoshi Hidaka +8 more
TL;DR: In this article, a newly synthesized isoquinolinesulfonamide, H-89 (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline-sulfoneamide), was shown to have a potent and selective inhibitory action against cyclic AMP-dependent protein kinase (protein kinase A), with an inhibition constant of 0.048 +/- 0.008 microM.
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KN-62, 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazi ne, a specific inhibitor of Ca2+/calmodulin-dependent protein kinase II
Hiroshi Tokumitsu,Takashi Chijiwa,Masatoshi Hagiwara,Akihiro Mizutani,Motomu Terasawa,Hiroyoshi Hidaka +5 more
TL;DR: Results suggest that KN-62 affects the interaction between calmodulin and Ca2+/CaM kinase II following inhibition of this kinase activity by directly binding to theCalmodulin binding site of the enzyme but does not affect the cal modulin-independent activity of already autophosphorylated (activated) enzyme.
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Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA.
Daisuke Kaida,Hajime Motoyoshi,Etsu Tashiro,Takayuki Nojima,Masatoshi Hagiwara,Ken Ishigami,Hidenori Watanabe,Takeshi Kitahara,Tatsuhiko Yoshida,Hidenori Nakajima,Tokio Tani,Sueharu Horinouchi,Minoru Yoshida +12 more
TL;DR: The natural product FR901464 and its methylated derivative, spliceostatin A are shown to inhibit in vitro splicing and promote pre-mRNA accumulation by binding to SF3b, a subcomplex of the U2 small nuclear ribonucleoprotein in the spliceosome.
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The newly synthesized selective Ca2+calmodulin dependent protein kinase II inhibitor KN-93 reduces dopamine contents in PC12h cells
Mariko Sumi,Kazutoshi Kiuchi,Tomohiko Ishikawa,Akira Ishii,Masatoshi Hagiwara,Toshiharu Nagatsu,Hiroyoshi Hidaka +6 more
TL;DR: Investigation of the inhibitory property of a newly synthesized methoxybenzenesulfonamide, KN-93, on CaMKII activity in situ and in vitro suggests that it inhibits DA formation by modulating the reaction rate of TH to reduce the Ca(2+)-mediated phosphorylation levels of the TH molecule.