Melanie P. Gygi

TL;DR: Using high-throughput affinity-purification mass spectrometry, BioPlex is used to identify interacting partners for 2,594 human proteins in HEK293T cells and reveals associations among thousands of protein domains, suggesting a basis for examining structurally related proteins.

TL;DR: With more than 56,000 candidate interactions, BioPlex 2.0 exceeds previous experimentally derived interaction networks in depth and breadth, and will be a valuable resource for exploring the biology of incompletely characterized proteins and for elucidating larger-scale patterns of proteome organization.

TL;DR: It is found that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity, by phosphorylation by AMPK at six previously unidentified regulatory sites.

TL;DR: In this article, it was shown that CASTOR1, a previously uncharacterized protein, interacts with GATOR2 and is required for arginine deprivation to inhibit mTORC1.

TL;DR: It is proposed that the exclusive association of U1 snRNP/SR proteins with RNAP II positions these splicing factors, which are known to function early in spliceosome assembly, close to the nascent pre-mRNA, so that these factors readily out-compete inhibitory hnRNP proteins, resulting in efficient splicesome assembly on nascentRNAP II transcripts.